Description

TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. TaqMan® MicroRNA Assays are:

  • Highly specific—quantitate only mature miRNAs, not precursors
  • Sensitive—conserve limited samples; requires only 1-10 nanograms of total RNA or equivalent
  • Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Our TaqMan® MicroRNA Assays catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.

Looking to Design Your Own Assay? Custom TaqMan® Small RNA Assays let you design your own TaqMan® Assay when a predesigned TaqMan® MicroRNA Assay is not offered. Simply submit your sequence information online, and we'll manufacture an assay to your specifications.

Availability & Sizes: Made-to-Order assays are available in four different scales and usually ship within two weeks. Inventoried assays are prepackaged in Small scale and are ready to ship. Inventoried assays may also be purchased in larger sizes by ordering as MTO.

Catalog # Availability # of RT/PCR Reactions
4427975InventoriedS: 50/150
4440885Made to OrderXS: 25/75
4440886Made to OrderS: 50/150
4440887Made to OrderM: 750/750
4440888Made to OrderL: 2900/2900

Specifications

General Specifications

Species:TaqMan® MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry
Assay Components:TaqMan® MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan® Assay (TaqMan® probe and PCR primer set).
Available Assays:Download: Complete list of TaqMan® MicroRNA Assays

Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

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