Description

TaqMan® Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold standard assay performance and data quality for pri-miRNA studies.

  • Highly specific – quantitiate miRNA transcription from a single genomic locus
  • Fast, simple, and scalable – two-step RT-qPCR provides high-quality results
  • A new perspective – measure miRNA expression at the gene level

TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic Assays are renowned, to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan® Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and Selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan® Pri-miRNA Assay and TaqMan® MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Catalog # Size # of Reactions/Concentration
4427012Small360/20X
4427013Medium750/20X
4427014Large2900/60X

Specifications

General Specifications

Pre-formulated assay (20x or 60x mix):
  • 2 unlabeled PCR primers (900 nM each final 1X reaction concentration; 150 nM final 1X reaction concentration
  • 1 FAM™ dye-labeled TaqMan® MGB probe (250 nM final 1X reaction concentration)
Documentation:On compact disc:
  • MSDS Formamide
  • MSDS Non-formamide
  • Protocol
  • Quick Reference Card
  • Understanding your Shipment
  • Assay Info File
  • Certificate of Analysis
  • Data Sheet
Printed copies of above documents
Assay Information File:Includes Sales Order Information, List of Contents (Assay ID, Part Number, Expiration Date, & Lot Number), Annotation Information (Gene Symbol, Gene Name, Entrez Gene ID, Transcript Accession & Location on Transcript) and Design Details (Context sequence, Amplicon size)
Tracking/Identification:
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes

Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Help Center

Order Support

Contact Order & Web Support, Search FAQs, View Order History and Track Your Order

Technical Support

Contact a Technical Application Scientist, Search Product Documents & FAQs and Instrument Maintenance & Repair Requests

Support Tools