Custom TaqMan® SNP Genotyping Assays provide a highly flexible technology for detection of polymorphisms within any genome. TaqMan® Assays have a simple workflow and provide the quickest way to generate genotyping data. They are ideal for genotyping applications including screening, association, candidate region, candidate gene, or fine-mapping studies.

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High Quality Assay Design Pipeline

Create your own assays by submitting confidential target sequences to our secure assay design pipeline using the Custom TaqMan® Assay Design Tool. This pipeline has generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data. These assays use TaqMan® minor groove-binding (MGB) probes for superior allelic discrimination, improved signal-to-noise ratios, and design flexibility. All human SNP genotyping assays are functionally tested on 20 unique DNA samples to ensure assay amplification and sample clustering. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.

A Complete Genotyping Solution

In addition to TaqMan® SNP Genotyping Assays, Life Technologies offers TaqMan® Genotyping Master Mix and superior real-time PCR instrument platforms for processing and analyzing all TaqMan® SNP Genotyping Assays. Our data analysis software automatically determines sample genotypes and generates a cluster plot to help you visualize data across samples or populations.

Part numbers for TaqMan® SNP Genotyping Assays

Catalog # - Human Catalog # - Non Human Size
43313494332077Small; 1,500 reactions; 40x
43320724332075Medium; 5,000 reactions; 40x
43320734332076Large; 12,000 reactions; 80x
Discrimination
5' nuclease assay to discriminate between 2 alleles of a specific SNP for use in genotyping studies.
Assay Components
Each assay contains:
  • 2 unlabelled PCR primers: forward and reverse (primers at 900 nM final concentration)
  • 1 VIC® dye – MGB-labeled probe detects the Allele 1 sequence (probes at 200 nM final concentration)
  • 1 6FAM™ dye – MGB labeled probe detects the Allele 2 sequence (probes at 200 nM final concentration)
Reactions (depending on scale)
  • 384-well plate: 1,500, 5,000, or 12,000 5-µL reactions
  • 96-well plate: 300, 1,000, or 2,400 25-µL reactions
Expiration Date   
5 years from date of manufacture.
Documentation    
Compact disk containing:
  • Assay Information File (AIF)
  • Datasheet (PDF)
  • Custom TaqMan® SNP Genotyping Assays Protocol (PDF)
  • Custom TaqMan® Assays Design and Ordering Guide (PDF)
  • Safety Data Sheet (PDF)
  • Understanding Your Shipment Document (PDF)
Assay Information File
Includes: sales order number, ship date, part number, product type, assay ID, lot number, assay mix concentration, primer & probe concentration, reporter dyes & quenchers, prier and probe oligonucleotide sequences, and other information.
Tracking/Identification
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes
Shipping and Storage ConditionsAssays ship at ambient temperature. Store at -15°C to -25°C upon arrival.

Citations