One-Step Real-Time RT-PCR Master Mixes

One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.

See All One-Step Real-Time RT-PCR Master Mixes

Which One-Step Real-Time RT-PCR Master Mix Is Right for You?

  Excellent Results for Everyday Applications Enabled for Fast Cycling When Time Counts Highest Overall Performance ( Sensitivity, Speed, and Tolerance to Inhibitors)
RNA-to-Ct™ 1-Step Kit
EXPRESS One-Step SuperScript® qRT-PCR Kits
TaqMan® Fast Virus 1-Step Master Mix
Detection Chemistry

TaqMan®

SYBR®

TaqMan®

SYBR®

TaqMan®

Sensitivity HighHighHigh
Cycling/Run Time120 minutes<60 min<60 min
Tolerant to PCR Inhibitors  yes
ApplicationsGene expression
RNA viral detection/quantitation
DNA viral/pathogen detection/quantitation
Multiplexing
Gene expression
RNA viral detection/quantitation
DNA viral/pathogen detection/quantitation
Multiplexing
Gene expression
RNA viral detection/quantitation
DNA viral/pathogen detection/quantitation
Multiplexing
UDG to Prevent DNA Carryover Contamination yes 
Reverse TranscriptaseArray Script UPSuperScript III Thermostable MMLV
ROX Reference Dye to Normalize Signal & Ensure Data Integrityyesversions with and withoutyes
Price per 20ul rxn$610 for 500 rxns$776 for 500 rxns $1562 for 1000 rxns
Format2 Tubes (20X RT Mix, 2X qPCR Mix)2 Tubes (10X RT Mix, 2X qPCR Mix)1 Tube (4X Mix)
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