One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.

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Which One-Step Real-Time RT-PCR Master Mix Is Right for You?

  Standard mode   Fast mode
Applications RNA viral detection/quantitation
DNA viral/pathogen detection/quantitation
Gene Expression
Multiplexing
RNA viral detection/quantitation
DNA viral/pathogen detection/quantitation
Gene Expression
Multiplexing
Detection Chemistry TaqMan®

SYBR®
TaqMan®
Sensitivity High High
Cycling/Run Time 120 minutes <60 min
Tolerant to PCR Inhibitors   yes
Reverse Transcriptase Array Script UP Thermostable MMLV
ROX Reference Dye to Normalize Signal & Ensure Data Integrity yes yes
Price per 20 µL rxn $1.13-1.58 $1.51-1.62
Format 2 Tubes
TaqMan® RT-PCR Mix (2x) and
TaqMan® RT Enzyme Mix (40x)
1 Tube (4X Mix)
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