SYBR® Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR® Green dye; everything needed for real-time PCR, except the sample and PCR primer pair.

Which SYBR® Green master mix is right for you?

  Gene Expression
(Fast or Standard mode)
Gene Expression
( Standard mode)
Applications Other applications:
DNA quantitation (pathogen detection)
CHiP
Other applications:
DNA quantitation (pathogen detection)
CHiP
Top selleryes  
Cycling/Run Time Standard (60 minutes) or
Fast (30 minutes)
60 minutes
Proprietary hot-start mechanism for exceptional specificityyes yes
Carryover contamination control Contains a blend of dTTP/dUTP that is compatible with AmpErase® UNG Only dU, compatible with UNG
Heat-labile UDG allows product to be stable 72 hrs pre and post qPCRyes  
Sensitivity 2 copies  
Instrument considerationsCompatible with all Applied Biosystems® real-time PCR instruments,  Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.  
Primer considerations

Recommended primer concentrations should be between 300-800 nM. 

 
Price / 20 µL Reaction $0.63-$1.03 $0.60-$0.98
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