SYBR® Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR® Green dye; everything needed for real-time PCR, except the sample and PCR primer pair.

Which SYBR® Green master mix is right for you?

  Gene Expression
(Standard)
Gene Expression
(Fast)
CHiP
(Standard)
Applications Gene expression
DNA quantitation (pathogen detection)
CHiP
Gene expression
DNA quantitation (pathogen detection)
Gene expression
DNA quantitation (pathogen detection)
CHiP
Top selleryes  yes
Mode
Cycling/Run Time
Gex/miRNA
GT
SYBR Gex
Pathogen, 1 step Gex
Standard
90 minutes
Gex
GTMM
Power
RNA to Ct
Fast
Standard (90 minutes) or Fast (30 minutes)
FAMM
GTXpress
SYBR Select
Fast Virus
Standard
90 minutes
Gex
GTMM
Power
RNA to Ct
SYBR GreenER™ dye for brighter signal yes  
Proprietary hot-start mechanism for exceptional specificity yes  
UDG for carryover contamination control yes  
Heat-labile UDG allows product to be stable 72 hrs pre and post qPCR yes  
Sensitivity 1 copy  
Instrument considerations Not recommended for 7900HT Real-time PCR instruments  
Primer considerations Only to be used with primer concentrations <200 nM for StepOne™/StepOnePlus™ Instruments  
Price / 20 µL Reaction$0.60-$0.98$0.54-$0.73
$0.60-$0.98
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