SYBR® Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR® Green dye; everything needed for real-time PCR, except the sample and PCR primer pair.

Which SYBR® Green master mix is right for you?

  For reliable performance For fast performance For best performance
Top selleryes  
Cycling modeStandard
(90 minutes)
(35 minutes)
Standard (90 minutes)
or fast mode (30 minutes)
SYBR GreenER™ dye for brighter signal  yes
Proprietary hot-start mechanism for exceptional specificity  yes
UDG for carryover contamination control yesyes
Heat-labile UDG allows product to be stable 72 hrs pre and post qPCR  yes
Sensitivity 2 copies1 copy
ApplicationsGene expression
DNA quantitation (pathogen detection)
Gene expression
DNA quantitation (pathogen detection)
Gene expression
DNA quantitation (pathogen detection)
Instrument considerations  Not recommended for 7900HT Real-time PCR instruments
Primer considerations Only to be used with primers with Tm ≥ 60°Only to be used with primer concentrations <200 nM for StepOne/StepOnePlus Instruments
Price / 20 µL Reaction$0.60-$0.98$0.68-$1.07$0.54-$0.73
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