Power SYBR® Green Master Mix
Obtain superior sensitivity and reproducibility without compromising any performance parameters in your real-time, quantitative PCR experiments.
- Highly sensitive DNA quantitation to enable low copy number detection
- Optimized formulation to offer improved consistency
- Compatible with existing Applied Biosystems® SYBR® Green reagent protocols
Detect as Few as 2 Copies of a Target Gene
Power SYBR® Green PCR Master Mix delivers highly sensitive nucleic acid quantitation, detecting as few as 2 copies of a target gene over a broad range of template concentrations. The newly optimized formulation contains highly purified AmpliTaq Gold® DNA Polymerase, LD to offer greater sensitivity. Additionally, our new formulation minimizes variation, ensuring consistency between experiments. Most importantly, Power SYBR® Green reagent-based PCR chemistry easily replaces SYBR® Green PCR Master Mix in Applied Biosystems protocols using the same set up and thermal cycling conditions.
Sensitivity without Compromise
Power SYBR® Green PCR Master Mix delivers superior sensitivity and reproducibility without compromising any other performance parameters such as specificity, wide dynamic range and uniformity. In addition, Power SYBR® Green PCR Master Mix provides high fluorescent signal strength for consistent detection of low copy numbers.
Power SYBR® Green PCR Master Mix contains a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR-related fluorescence fluctuations. Normalizing with an internal passive reference minimizes well-to-well variability that results from a variety of causes, such as pipetting error and sample evaporation.
Ready to Use
Power SYBR® Green PCR Master Mix provides greater flexibility, reduces experimental design time and requires fewer reagents. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR. AmpliTaq Gold® DNA Polymerase, LD is a hot start PCR enzyme provided in an inactive state allowing for room temperature set up and minimizing non-specific amplification.