Taq Man® Real-Time PCR Master Mixes

TaqMan® Master Mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable TaqMan® Assay-based real-time PCR. Just add your sample and TaqMan® Assay, and start your reactions.

See all TaqMan® master mixes ›


Which TaqMan® master mix is right for you?

  Gene Expression
(Standard mode)
Gene Expression
(Fast mode)
  Genotyping
(Standard mode)
Genotyping
(Fast mode)
 
Application examples Gene expression for mRNA & small RNA Gene expression for mRNA & small RNA   SNP genotyping,
Mutation detection,
CNV
SNP genotyping,
Crude lysate samples
Cycling/Run Time
1:20
0:40
  1:20
0:50

UDG to prevent DNA carryover contaminationyesyes      
ROX reference dye to normalize signal & ensure data integrityyesyes   yes yes
Universal cycling for assay to assay consistencyyesyes   yes yes
Benchtop stability24 hr72 hr   24 hr 24 hr
Tolerant to PCR inhibitors yes     yes
Price / 20 µL Reaction $0.62-$0.79 $0.81-$0.95   $0.58-0.85 $0.60-0.78
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*While TaqMan® Universal Master Mix II can be used for all applications, TaqMan® Genotyping Master Mix has been specifically designed and will provide the best performance for genotyping applications (e.g. CNV, DME & SNP).

Other master mixes

TaqMan® PreAmp Master Mix is designed to facilitate analysis of limited-quantity samples, including single cells. Use the mix to expand small amounts of cDNA by pre-amplification without introducing amplification bias to the sample. Pre-amplification enables you to stretch your precious sample for use in many more real-time PCR reactions than you could otherwise run.

TaqMan® Genotyping Master Mix is specifically formulated for detection of copy number, DME’s, and SNPs. Use this mix for the best performance for genotyping applications.