Life Technologies SuperScript® III Reverse Transcriptase is the most widely used brand of enzyme for cDNA synthesis, due to the proven ability to deliver reliable and consistently high yields of fully representative full length cDNA.

Which Reverse Transcription Enzyme Is Right for You?

Standard cDNA Synthesis General-Purpose cDNA Synthesis High Performance cDNA Synthesis Standard High Temperature cDNA For High Temperature cDNA Synthesis
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  Invitrogen MMLV Invitrogen SuperScript® II Invitrogen SuperScript® III
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Invitrogen AMV-Cloned Invitrogen ThermoScript™
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Optimal Reaction Temp. 37°C 42°C 50°C 55°C 65°C
Max cDNA Length Up to 7 kb Up to 12.3 kb Up to 12.3 kb Up to 12.3 kbUp to 12.3 kb
Sensitivity 1 ng - 5 µg Total RNA 10 pg - 5 µg Total RNA 10 pg - 5 µg Total RNA 10 pg - 5 µg Total RNA 10 pg - 5 µg Total RNA
Reduced RNase H Activity  (high)  (high)  (moderate)
RT-PCR Kits Format
First-strand Synthesis Kits Format

Learn more About Reverse Transcriptase Enzymes for your Application Workflow