Selecting the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of high-quality, full-length cDNA. We offer a range of stand-alone reverse transcriptases, optimized to generate cDNA for a variety of research needs.

Which stand-alone reverse transcriptase is right for you?

Value choice Standard choice Preferred choice
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  MMLV SuperScript® II
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SuperScript® III
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Optimal reaction temp. 37°C 42°C 50°C
Half-life @ 50oC 3.5 min 6.1 min 220 min
Max cDNA length Up to 7 kb Up to 12.3 kb Up to 12.3 kb
Sensitivity 1 ng–5 µg total RNA 10 pg–5 µg total RNA 10 pg–5 µg total RNA
Reduced RNase H activity  (moderate)  (high)