SuperScript® II Reverse Transcriptase (RT) is an engineered version of M-MLV reverse transcriptase with reduced RNase H activity and increased thermal stability. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. The structural modification of this enzyme eliminates degradation of RNA molecules during first-strand cDNA synthesis gives SuperScript® II RT the superior performance characteristics below.

Ordering information

Figure 1. Superior sensitivity of SuperScript® Reverse Transcriptase. Serial dilution of HeLa cell tRNA at 1 μg, 1 ng, 100 pg, and 10 pg (lanes 1 through 4 respectively) were reverse transcribed using the manufacturer’s instructions for each enzyme. 

Transcript sensitivity

With over 50,000 citations, reviews, and publications, SuperScript® II is the most widely used RT for cDNA synthesis. One of the common uses for SuperScript® II is gene expression analysis [1]. For gene expression studies, the ideal RT will efficiently reverse transcribe even the least abundant mRNAs or low copy RNA within a total RNA pool. SuperScript® II can reverse transcribe 532 bp and 1.1 kb transcripts from as little as 100 pg of input tRNA. In contrast to ProtoScript® II (NEB), SuperScript® II has 10-fold increase in transcript sensitivity. For even higher sensitivity, SuperScript® III can achieve a signal from 10 pg of input tRNA. 

Figure 1. Superior sensitivity of SuperScript® Reverse Transcriptase. Serial dilution of HeLa cell tRNA at 1 μg, 1 ng, 100 pg, and 10 pg (lanes 1 through 4 respectively) were reverse transcribed using the manufacturer’s instructions for each enzyme. 


Transcript length

Another critical feature of reverse transcriptases—especially for cloning or cDNA library applications—is the ability to reverse transcribe full-length mRNA. Without efficient extension of RNA during the RT reaction, portions of the transcript may be lost for analysis. In a time-course RT reaction, SuperScript® II was effective in the reverse transcription of 0.24 to 9.5 kb RNA ladder at 15 min as compared to MMLV, GoScript™ (Promega), MMLV H– (Promega) and Sensiscript® (Qiagen) RTs.

Figure 2.  Reverse transcription of 0.24–0.5 kb RNA ladder transcript. RNA ladder was reverse transcribed using the manufacturer’s instructions for each enzyme.


Featured products based on SuperScript® II Reverse Transcriptase

Ordering Information

Sku Name Size Price Qty
18064022 SuperScript® II Reverse Transcriptase 2000 units USD 83.00
18064014 SuperScript® II Reverse Transcriptase 10,000 units USD 281.00
18064071 SuperScript® II Reverse Transcriptase 4 x 10,000 units USD 1,000.00
11904018 SuperScript® First-Strand Synthesis System for RT-PCR 50 reactions USD 394.00
10928034 SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase 25 reactions USD 200.00
10928042 SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase 100 reactions USD 584.00
11922010 SuperScript® One-Step RT-PCR System for Long Templates 25 reactions USD 227.00
11922028 SuperScript® One-Step RT-PCR System for Long Templates 100 reactions USD 634.00