Perform both cDNA synthesis and PCR amplification in a convenient one-step formulation. Our One-Step RT-PCR Kits contain dNTPs and a mixture of reverse transcriptase and thermostable hot-start DNA polymerase in an optimized reaction buffer. Just add your RNA sample and gene-specific primers, and start your reactions.

What is the difference between one-step and two-step RT-PCR?

Which one-step RT-PCR kit is right for you?

Standard choice for RT-PCR Preferred choice for RT-PCR Preferred choice for RT-PCR with high fidelity
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  SuperScript® One-Step RT-PCR System with Platinum® Taq DNA polymerase SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA polymerase SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity
Reverse transcriptase SuperScript® II SuperScript® III SuperScript® III
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Optimal reaction temp. 42°C 55°C 55°C
Max cDNA length 3 kb 4.5 kb 10 kb
Sensitivity 10 pg–1 µg Total RNA 0.01 pg–1 µg Total RNA 1 pg -1 ug Total RNA
PCR enzyme Platinum® Taq DNA polymerase Platinum® Taq DNA polymerase Platinum® Taq High Fidelity DNA polymerase