Choosing the correct RT-PCR kit for cDNA synthesis is critical to obtaining high yields of cDNA to improve the sensitivity of your RT-PCR assays. Life Technologies offers a wide range of Reverse Transcriptase enzymes optimized for generating cDNA for one and two step RT-PCR. Choose from a variety of enzyme types and formats, including SuperScript® III, SuperScript® II and ThermoScript.

Select a Reverse Transcriptase

Which RT-PCR Kit Is Right for You?

Standard RT-PCR for Smaller Templates Standard RT-PCR for Long Templates High Performance RT-PCR High Performance RT-PCR with High Fidelity High Temperature RT-PCR with High Fidelity
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  Invitrogen SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase Invitrogen SuperScript® One-Step RT-PCR System for Long Templates Invitrogen SuperScript® III One-Step RT-PCR System with Platinum® Taq Invitrogen SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity Invitrogen ThermoScript™ RT-PCR System plus Platinum® Taq DNA Polymerase High Fidelity
RT Enzyme SuperScript® II SuperScript® II SuperScript® III SuperScript® III Invitrogen ThermoScript™
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Optimal Reaction Temp. 45°C 45°C 55°C 55°C50°C
Max cDNA Length 3 kb 9 kb 200 bp - 4.5 kb 300 bp - 10 kb 100 bp - 12 kb
Sensitivity 10 pg - 1 µg Total RNA 10 pg - 1 µg Total RNA 0.01 pg - 1 µg Total RNA 1 pg - 1 µg Total RNA 10 pg - 5 µg Total RNA
One Step or Two Step One Step One Step One Step One Step Two Step