DNA Helix

Plant genome sequencing allows researchers to uncover the genetic makeup of plants. This is fundamental not only to answering basic research questions but also to designing the next-generation of crop plants. Sequencing plant genomes is becoming easier and more cost effective, even for de novo sequencing projects aimed at obtaining the primary genetic sequence of your plant species of interest. Life Technologies has an extensive portfolio of sequencing instruments, reagents and analysis software to get you there faster and more reliably and accurately.

What is de novo plant sequencing?

Plant de novo sequencing refers to sequencing and assembling a plant genome without any reference genome sequence. Next-generation sequencing technologies sequence millions of bases in a single round. This leap in throughput allows researchers to move beyond model organisms and gain an understanding of all plant genomes—a critical step in unraveling the complexity of plants.

Plant de novo sequencing solutions enabled by Life Technologies

Powered by Ion semiconductor sequencing, the Ion Personal Genome Machine™ (PGM™) System and the Ion Proton™ System deliver the fastest run times at the most affordable price of any next-generation sequencers. These dramatic improvements in sequencing technology are changing the way plant scientist look at genomics and are paving the way for the next wave of remarkable agriculture discoveries.

The Ion Torrent benchtop sequencers
For de novo sequencing of any plant genome For de novo sequencing of plant genomes up to 1 Gb

The Ion Proton™ Sequencer is the first benchtop next-generation sequencer to offer fast, affordable genome and exome sequencing in plants. Also powered by Ion semiconductor sequencing technology and offering a similar single-day workflow to the Ion PGM™ System, the Ion Proton™ System is poised to bring the genomics revolution to every single economically important plant species, offering:

  • The most affordable genome sequencer
  • High-throughput Proton I Chip (166 million wells) and Proton II Chip (660 million wells)
  • 2-hour run time
  • Workflow that allows you to sequence a plant genome in a single day
  • High accuracy
  • 200 bp read length

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The Ion Personal Genome Machine™ (PGM™) Sequencer makes next-generation sequencing more accessible to plant scientists across the globe, offering scalability, simplicity and speed:

  • The fastest run times: 90 minutes for 100 base sequencing on the Ion 314™ Chip
  • The most affordable price
  • High accuracy
  • 200 bp read length (up to 400 baselong reads)
  • 11 million wells, up to 1 Gb output on the Ion 318™ chip
  • One instrument, multiple applications: de novo sequencing, transcriptome sequencing, genotyping by sequencing, resequencing, etc.
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