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Genotyping by real-time PCR, whether for the confirmation of single nucleotide polymorphisms (SNPs) or as a screen for panels of markers in hundreds or even millions of samples, is a well-known and demonstrated approach used widely in both research and industrial settings. Life Technologies is the leader in providing real-time PCR instruments, TaqMan® Assays, and reagents that generate reliable results for validation and screening.

Flexible, high-throughput genotyping by real-time PCR with the QuantStudio™ 12K Flex Real-Time PCR System and Custom TaqMan® SNP Genotyping Assays

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QuantStudio™ 12K Flex Real-Time PCR System

  • Flexibility—five interchangeable blocks to fit your needs
  • 110,000 genotypes per workday without robotics
Other instruments available to meet your throughput needs and budgets.
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Custom TaqMan® SNP Genotyping Assays

Custom TaqMan® SNP Genotyping Assays provide a highly flexible technology for detection of polymorphisms within any genome. Create your own assays by submitting confidential target sequences to our secure assay design pipeline using the Custom TaqMan® Assay design tool. This pipeline has successfully generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data.

Custom TaqMan® Assay Manufacturing & Plating Service

Whether it’s zygosity analysis, or special manufacturing modifications such as a specific dye, assay volume/concentration, or plating format, Life Technologies can address your special needs. Contact your sales representative to discuss your needs.

Overview of workflow for genotyping by real-time PCR

Publications using Custom TaqMan® SNP Genotyping Assays

TitleAuthorsAddressLink
Mapping of the oat crown rust resistance gene Pc91. McCartney CA, Stonehouse RG, Rossnagel BG, Eckstein PE, Scoles GJ, Zatorski T, Beattie AD, Chong J  Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada.View
A fluorogenic 5’ nuclease (TaqMan) assay to assess dosage of a marker tightly linked to red skin color in autotetraploid potato. De Jong WS, De Jong DM, Bodis M Department of Plant Breeding, Cornell University, Ithaca, NY 14853-1901, USA.View
Discovery and characterization of single nucleotide polymorphisms in Chinook salmon, Oncorhynchus tshawytscha. Clemento AJ, Abadía-Cardoso A, Starks HA, Garza JC. Southwest Fisheries Science Center, National Marine Fisheries Service, Santa Cruz, CA 95060, USA.View
SNP genotyping by the 5’-nuclease reaction: advances in high-throughput genotyping with nonmodel organisms. Seeb JE, Pascal CE, Ramakrishnan R, Seeb LW. School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, USA.View
A nanoliter fluidic platform for large-scale single nucleotide polymorphism genotyping. Roberts DG, Morrison TB, Liu-Cordero SN, Cho J, Garcia J, Kanigan TS, Munnelly K, Brenan CJ.BioTrove Inc., Woburn, MA 01810, USA.View
Simplex and duplex event-specific analytical methods for functional biotech maize. Lee SH, Kim SJ, Yi BY.  GMO Testing Laboratory, Experiment Research Institute of National Agricultural Products Quality Management Service, Seoul, South Korea.View
Quantitative real-time PCR assay for determining transgene copy number in transformed plants.Biotechniques. 2001 Jul;31(1):132-4, 136-40. Ingham DJ, Beer S, Money S, Hansen G. Syngenta, Research Triangle Park, NC, USAView

Ordering information

TaqMan ® GTXpress™ Master Mix and TaqMan® Sample-to-SNP™ Kit

Product

Cat. No.

Size

TaqMan® GTXpress™ Master Mix

4403311

400 reactions

4401892

4,000 reactions

4401890

2,000 reactions

4401857

40,000 reactions

TaqMan® Sample-to-SNP™ Kit

4403081

200 mL sample prep, 10 mL PCR

4403083

20 mL sample prep, 10 mL PCR

4403087

20 mL sample prep, 50 mL PCR

4403313

5 mL sample prep, 1 mL PCR


Custom TaqMan® SNP Genotyping Assays

Product

Cat. No.

Size

Custom TaqMan® SNP Genotyping Assays

4332077

Small: 1,500 reactions, 40x

4332075

Medium: 5,000 reactions, 40x

4332076

Large: 12,000 reactions, 80x

Order Custom TaqMan® SNP Genotyping Assays now


TaqMan® OpenArray® Genotyping Plates

Plate Format# of Samples/PlateMin. OrderTotal # of Samples/Min. Order
16 assays144101,440
32 assays9610960
64 assays4820960
128 assays2440960
192 assays1660960
256 assays1280960
Learn about the TaqMan® OpenArray® Genotyping Plates
Configure a TaqMan® OpenArray® Genotyping Plate
TaqMan® Custom Plating Service for 96-/384-well plates
Learn about our TaqMan® Custom Plating Service

Special manufacturing request

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