Featured ELISA kit categories
High-quality ELISA kits designed for researchers studying neurological disorders or neuroscience-related research.
Phosphorylation ELISA kits are designed to deliver accurate, sensitive, and fast protein quantitation of total and phosphorylated, modified, or cleavage site-specific proteins.
Extensive portfolio of over 700 cytokine, chemokine, interleukin, and extracellular ELISA kits, covering a broad range of targets in several species and various sample types.
Colorimetric and near infrared detection kits for simple and convenient methods for the quantification of intracellular protein levels in whole cells.
Watch ELISA kits in action
ELISA kit features
The extensive ELISA kit portfolio ranges from high-quality neurobiology-related and phospho-specific ELISA kits to a broad menu of cytokine/chemokine ELISA kits.
ELISA kits help provide accurate and sensitive and consistent results. Each target protein is researched and the kits are calibrated to provide physiologically relevant sensitivity. In addition, kits are validated using common sample types, including serum, plasma and cell culture supernatant. Cell lysates are used to validate kits that detect signaling proteins or phosphorylation.
Our ELISA kits must meet rigorous quality-control specifications and are manufactured in an ISO facility with stringent quality controls to help ensure excellent quality and reproducibility.
Advantages of these ELISA kits:
- Broad menu of over 800 targets
- Optimized for sensitive, accurate, and consistent performance
- Thorough instructions to complete protocol in 2.5 to 4 hours (varies by kit)
- Validated for typical sample types (e.g., serum, plasma, supernatant, lysates)
ELISA kits typically include:
- Antibody-coated 96-well plate
- Primary detection antibody (typically biotinylated)
- Secondary detection reagent (usually streptavidin-HRP)
- Diluent buffers
- Wash buffers
- Substrate and stop solutions
- Plate covers
Most kits are effective for several sample types (e.g., serum, plasma, and cell culture supernatant) or cell lysate, tissue homogenate, urine, and cerebrospinal fluid (CSF), as appropriate to the target. Kits are available for use with human, mouse, rat, monkey, bovine, and porcine (swine) samples. Several ELISA kits are designed for use with multiple species.
Fast and easy, 4-hour Novex® ELISA kit protocol. Capture antibodies are precoated on the bottom of a 96-well plate. The sample or standard is added to the wells and incubated to allow target proteins to bind. The wells are then washed to remove unbound material, and the detection antibody is added and incubated to form a sandwich around the protein of interest. HRP conjugate is then added and incubated to allow binding via a biotin–streptavidin interaction. Next, the chromogen substrate for HRP is added, and the subsequent enzymatic reaction turns the solution blue. Finally, the reaction is stopped, turning the solution yellow in proportion to the amount of target protein in the sample. Absorbance is read in a microplate reader at 450 nm.
General schematic of phosphoELISA™ protocol. Capture antibodies are precoated on the bottom of a 96-well plate. The cell extract or standard is added to the wells and incubated to allow target proteins to bind. The wells are then washed to remove unbound material, and the detection antibody is added and incubated to form a sandwich around the protein of interest. HRP anti-rabbit antibody is then added and incubated to allow binding to the rabbit-derived detection antibody. Next, the chromogen substrate for HRP is added, and the subsequent enzymatic reaction turns the solution blue. Finally, the reaction is stopped, turning the solution yellow in proportion to the amount of target protein in the sample. Absorbance is read in a microplate reader at 450 nm.
Novex® ELISA kits provide reproducible results from lot to lot. Individual production lots (A–K) of the Novex® IFN-γ Human ELISA Kit were analyzed using 4 concentrations of control target protein (C1–C4). All 11 lots provided uniform sensitivity and low lot-to-lot variation (<20%).
phosphoELISA™ Kit standards are comparable to natural samples. Recombinant standards are tested against cell lysates to ensure correct measurement values of natural samples. Note that the standard is closely aligned to the natural sample used.
High specificity of the STAT3 [pY705] phosphoELISA™ kit: peptide competition. Peptide blocking is performed on each kit to confirm specificity of the phosphorylation site. The phosphorylated tyrosine 705 blocks the ELISA signal, but the nonphosphorylated peptide sequence and another phosphopeptide do not.
High specificity of the phosphoELISA™ kit: no cross-reactivity. HEL cell lysates were incubated with the capture antibody used in the STAT5a [pY694] ELISA (lane 2). An antibody specific for STAT5a and STAT5b was used as a positive control (lanes 1 and 4). IgG beads were used as a negative control (lane 3). The capture antibody recognizes the a isoform of STAT5 but not the b isoform. Thus, the STAT5a [pY694] ELISA does not cross-react with the STAT5b protein.
The expression of α-synuclein in various cell lines, detected by α-synuclein ELISA. The α-synuclein ELISA kit is specific for measuring α-synuclein and is consistent with western blotting (inset). The blot was probed with α-synuclein rabbit polyclonal antibody and developed using an alkaline phosphatase–conjugated anti–rabbit IgG followed by a chemiluminescent substrate and autoradiography.
Correlation between recombinant and natural Hu Tau. Natural human tau protein was serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve obtained using the Tau (Total) Human ELISA Kit. The closely aligned values of the natural and recombinant proteins indicate that the standard accurately reflects natural human tau content in samples.
Figure 7. Correlation between recombinant and natural human vascular endothelial growth factor (hVEGF). Natural hVEGF was serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve. The closely aligned values of the natural and recombinant proteins indicate that the standard accurately reflects natural human VEGF content in samples.
Figure 8. Representative standard curves for human IL-8 ELISA kits. Standard curves generated using the Novex® IL-8 Human ELISA Kit (normal) and the Novex® IL-8 Human Ultrasensitive ELISA Kit (ultrasensitive).
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For Research Use Only. Not for use in diagnostic procedures.