Western Blot Workflow Selection Guide
Western blotting is a method that remains a fundamental and integral part of research programs involving protein biology.
In this technique, protein samples derived from biological fluids or by lysis of cultured cells are first separated by gel electrophoresis and transferred onto PVDF or nitrocellulose membrane. Then proteins of interest are probed with specific antibodies that are tagged (most commonly with HRP enzyme), allowing those protein bands on the blot to be detected and imaged after tag-specific substrates are applied.
We offer a wide range of reagents, kits, gels, membranes, antibodies, and equipment to facilitate every step of western blot analysis. Use this product selection guide to choose the appropriate product for your western blotting needs.
Precast uniform and gradient gels for SDS-PAGE with long shelf-life, fast run-time, and excellent resolution.
Designed for both fast Coomassie staining of protein gels and for rapid semi-dry transfer of proteins from gel to membrane.
The automated western processing device that is hands-free, reproducible, and compatible with all western detection protocols.
Western Blot Workflow
- Protein transfer
- Transfer buffers
- Blocking buffers
- Wash buffers
- iBlot® 2 Dry Blotting System
- Pierce™ Power System
- Primary antibodies
- Secondary antibodies
- Streptavidin conjugates
- Antibody labeling kits
- WesternDot™ immunodetection kits
- Alexa Fluor® secondary antibodies
For Research Use Only. Not for use in diagnostic procedures.