Whether you just need a quick visual confirmation or require a highly-sensitive stain to detect low-abundance proteins, we offer a variety of easy-to-use, effective protein stains for both in-gel and on-membrane detection. Check out our comprehensive collection of stains including Coomassie dye-based, silver, fluorescent, tagged fusion protein, specialty, and multiplexed proteomics protein gel stains.

Choose the protein staining method most suitable for your experiments

  Coomassie Staining Silver Staining Fluorescent Protein Staining

Sensitivity

25 ng

0.5 ng

0.5 ng

Ease of use

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Mode of action

In acidic buffer conditions, coomassie dye binds to basic and hydrophobic residues of proteins, changing from dull reddish-brown to intense blue.

Silver ions interact and bind with with carboxylic acid groups ( Asp and Glu), imidazole (His), sulfhydryls (Cys), and amines (Lys). Silver ions are reduced to metallic silver, resulting in brown-black color.

Most fluorescent stains involve simply dye-binding mechanisms rather than chemical reactions that alter protein functional groups.

Detection

Visual

Visual

Compatible imaging system

Compatibiltity with downstream applications

MS and sequencing compatible

Certain formulations are MS compatible

Most stains are MS compatible

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*The number of steps includes multiple gel washes, solution changes and destaining steps.

Featured additional staining solutions

Preferentially detect histidine-tagged (His-tagged) recombinant fusion proteins in polyacrylamide gels

Includes in-gel phospho- and glycoprotein detection and on-membrane reversible protein staining kits

Pierce-Power-Stainer