Enzyme Substrate Profiling

ProtoArray® Human Protein Microarrays allow for easy Identification of kinase, methyltransferase, and ubiquitin ligase substrates, enabling researchers to discover and identify new targets in as little as one day.

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Easily Profile Enzyme Substrates

ProtoArray® Human Protein Microarrays are an excellent method to identify enzyme substrates. Using this method you can profile your purified enzyme or cell lysates against 9,000 functional proteins and identify possible targets. Since the identity of every protein on the array is known, you can immediately identify any proteins that your protein modifies. The protocol has been adapted to study numerous modifications including phosphorylation, methylation, ubiquitination, SUMOylation, NEDDylation, and nitrosylation.

Unlike other systems for analyzing protein modification, the enzyme substrate profiling application allows researchers to discover and identify new targets in as little as one day. In addition to testing enzyme activity, inhibitors and other compounds can be added to the assay to profile there effect on the enzyme substrate profile. This technique allows researchers to rapidly assess the specificity of drugs on enzymes.

The ProtoArray® Enzyme Substrate Profiling application is available either as a custom service or as a product.
Enzyme substrate profiling
Figure 1. Profiling enzymatic activity.

Selected References

ProtoArray® Enzyme Substrate Profiling Service
  • Pre-service Consultation — Receive a consultation session with our experienced service scientists to optimize your experimental design
  • Post-service Comprehensive Report — A complete report detailing the profiling experiment and results will be delivered to you in approximately 4 weeks
  • Technical Support — Our responsive support and dedicated staff will be available to you through the completion of your project

Learn More About our Enzyme Substrate Service with ProtoArray®v5.0.

Ordering information

Sku Name Size Price Qty
PAH0525101 ProtoArray® Human Protein Microarray 1 array USD 1,025.00
PAH05251020 ProtoArray® Human Protein Microarray, Discovery Study Bundle 20 arrays USD 20,500.00
PA055 ProtoArray® Blocking Buffer Kit 1 kit USD 83.50
PV4379 Biotin-Ubiquitin 10 µg USD 237.44
PV3304 MAPK14 (p38 α) Recombinant Human Protein 10 µg USD 379.00
S32357 Streptavidin, Alexa Fluor® 647 Conjugate 0.5 mL USD 219.00