NovaBright™ Chemiluminescent Beta-galactosidase Reporter Gene Assays

NovaBright™ beta-galactosidase reporter gene assay for mammalian or yeast cells combines direct cell lysis with the rapid ultrasensitive detection of beta-galactosidase reporter enzyme in a homogenous assay format. The kit uses the combination of Galacton-Star® substrate and Sapphire-II™ enhancer to give sensitivity and dynamic range unmatched by colorimetric methods. The kit is formatted with two alternative reaction buffers for the lysis of mammalian or yeast cells.

NovaBright™ Ordering Information

NovaBright™ β-galactosidase Reporter Gene Assays

NovaBright™ β-galactosidase Reporter Gene Assays

Sensitivity of NovaBright™ β-galactosidase Reporter Gene Assay Detection. NovaBright™ β-galactosidase Reporter Gene Assays were performed with the indicated amount of purified β-galactosidase diluted in PBS. Assays were measured on a microplate luminometer.
 NovaBright™ β-galactosidase Reporter Gene Assays are available for yeast or mammalian cells and combine direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase.

  • Homogeneous assay is automation-friendly
  • Persistent glow readout remains constant for 45 to 90 minutes
  • Sensitivity from picogram to nanogram amounts of reporter

 

Workflow

NovaBright™ Beta-gal Report Gene Assay Workflow

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