Bacterial Expression

Reliable Prokaryotic Systems to Fit Every Expression Scale

Producing large quantities of protein quickly and economically often requires using a bacterial host organism. Life Technologies offers a variety of systems for expression of recombinant proteins in Escherichia coli (E. coli). 

Methods

Routine T7 Expression

Ideal for nontoxic, recombinant protein expression in E. coli—pRSET and Gateway® pDEST™ vectors

Highest Levels of Protein Expression

Our strongest promoter to achieve maximum protein production—Champion™ pET

Regulated Expression

Tightly control expression of toxic proteins and optimize protein solubility—pBAD

Reagents

Competent Cells for Expression

Comprehensive selection of reliable and efficient BL21 strains—One Shot® E. coli

Bacterial Culture

Bacterial culture products, growth media, and reagents

Resources