Mammalian Expression

Broad Portfolio Solves Your Expression Challenge

Producing a functional mammalian protein often necessitates using a mammalian expression host to ensure appropriate posttranslational modifications (e.g., disulfide bonds, glycosylation, phosphorylation). Life Technologies offers many flexible options for mammalian protein expression, including a wide selection of TOPO® and Gateway® expression vectors and mammalian cell culture products.

Methods

Constitutive Expression

High-yield mammalian protein expression—pcDNA™ 3.3 vectors

Inducible Expression

Ideal for toxic proteins and controlling expression levels—T-REx™ system

Viral Delivery

Ideal for difficult-to-transfect cell lines—ViraPower™ system

Large-Scale Protein Production

Rapid, high-yield, transient protein production using mammalian cells—Expi293™ and FreeStyle™ Protein Expression Systems

Stable Cell Line Development

Create stable, isogenic mammalian cell lines—Flp-In™ and Jump-In™ systems

Applications

Membrane Proteins

Generate functional, soluble GPCRs in mammalian cells—MembranePro™ system

Fluorescent Proteins

Fusion proteins for labeling and imaging—GFP and Vivid Colors™ systems

Mammalian Cell Culture

Gibco® cell culture products, growth media, and reagents