The Flp-In™ System allows stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase–mediated DNA recombination at the FRT site.
Life Technologies offers a selection of Flp-In™ products, including expression vectors and systems, as well as parental cell lines with a stably integrated FRT site. Designed for rapid generation of stable cell lines, these products ensure high-level expression of your protein of interest from a Flp-In™ expression vector.