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Life Technologies offers a range of protein expression systems and tools that are easy to use,deliver high protein yields for every downstream application and allow you to get there quickly. Creating your gene, purifying your plasmid and delivery is simplified with the selection tools and educational videos.

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Protein Expression Workflow

geneart-assemblygeneart-synthesisoneshotbenchpropurelinkexpi293lipopichiabacecoli

Selecting a Host System for Protein Expression & Production

When selecting a host system for expressing protein there are various considerations. From post translational modifications, equipment available to how quickly you need it, we have mapped out the hosts to help you make the choice. For each host system we have a information that provides you with all our applicable products along the we offer, allowing you to make the right choice based on your needs and budget.

  Do you need modifications? How quickly do you require protein? What capabilities do you have to support expression (lab equipment & resources)?
 NoneSomeCompleteDaysWeeksMonthsNoneSomeMany
Mammalian         
Bacteria         
Yeast         
Insect         

 

Mammalian Expression

Vector selection Cloning Transform in E. Coli Plasmid Purification Transfection Expression Purification Analysis

Determined by promoter and terminator

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Determined by vector choice

- or -

GeneArt® Gene Synthesis

Recommend DH5alpha TOP10 strain

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Could be maxi, mega or giga

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Depends on speed

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Media based on expression system choice: 293 or Freestyle

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Affinity based with tag or native

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Analysis, & Assay

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Bacterial Expression

Vector selection Cloning Transform in E. Coli Plasmid Purification Transform Optimization of Clone Scale & Purify Analysis

Any vector with a T7 promoter

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Choose any cloning method

- or -

GeneArt® Gene Synthesis

Recommend TOP10 strain but can choose from subcloning products

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Mini prep kits

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Transform into any expression strain

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TB or LB

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TB, LB or MagicMedia

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& resin purification

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Analysis, & Assay

choose

Yeast Expression

Vector selection Cloning Transform in E.Coli Plasmid Purification Transform Into Yeast Clone Selection Express Purification Analysis

Does protein have a secretion signal

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Yes? use Pichia®

Clone with restriction enzymes

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One Shot® TOP10 Electrocomp™ E. coli

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Midi or maxi prep depending on yield requirement

Yeast strain

Zeocin

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Media

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With secretion sometimes not necessary & affinity for tag

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Analysis & Assay

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Insect Expression

Vector selection Cloning Transform in E.Coli Plasmid Purification Transform Into Bac Purify Bacmid Transfect Amplify/ Express Analysis

Choose TOPO or Gateway vector

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Select  TOPO or Gateway for Cloning

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Recommend DH5alpha TOP10 strain but can choose from subcloning CVC

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Mini prep kits

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Transform into DH10Bac

Midi or maxi prep- depending on yield requirement

Transfection with CellFectin II

SF9 & SF21

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Analysis, & Assay

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How-To Videos

Cloning
 
Transformation
   
   
         
Plasmid Purification
 
Expression
   
   
         
 
   
         
 
   
 

"Meet the Inventor" Series

Clone: 20% Off GeneArt® Expression-Optimized Genes

Improve protein expression up to 100-fold with GeneArt® expression-optimized genes.

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