Tools for Optimizing Expression
Nearly all molecular biology experiments start with cloned DNA. The gene can be a starting point for protein expression, structural analysis or a functional experiment.
Researchers may derive their DNA from PCR done on a natural source or purchase it from a premade clone collection. Buying the gene from an ORF collection is the fastest way if the gene of interest is available. However, you have to rely on preexisting material. Modifying these constructs is time consuming and costly. Gene synthesis offers the opportunity to design every construct from scratch-tailored for the respective experiment. Starting point is any sequence information. This offers maximum flexibility to get the gene of choice within 8–10 business days, 100% sequence verified, cloned into a vector of choice.
Synthesis of an optimized gene further enhances protein expression levels. In addition, optimized genes are resistant to RNA knock down.
Protein Expression Optimization
|100% sequence verified clone available in less than a week||Protein expression with no gene design constraints||Improved protein expression levels through gene optimization|
|Key Features||Fastest way to obtain a sequence verified clone||Full service that delivers validated genes with no limitation in sequence design||Full service that delivers validated genes with improved expression yields & no limitation in sequence design|
|Time to get to expression||1 week||6–15 days to receive clones ready for use||6–15 days to receive clones ready for use|
|Gene Size||1||up to 20 kb||up to 20 kb|
|Number of Fragments||1||N/A||N/A|
|What is delivered||Glycerol stock of uORF entry clone. ORF card data available online||Synthetic wild type gene 5 µg lyophilized DNA cloned in GeneArt® standard vector Quality Assurance Documentation including sequence verification||Optimized Gene 5 µg lyophilized DNA cloned in GeneArt® standard vector Quality Assurance Documentation including sequence verification|
|Choice of Expression Vectors||Any Gateway® Destination vector||Any vector||Any vector|
|Improvement of expression yields||N/A||N/A||Up to 100 fold|
|Free webtool for search & design||Yes||Yes||Yes|
|Price per unit||as low as $49/ clone||$0.35/bp (0.5–3kb);
$169 up to 500 bp
$169 up to 500 bp
|Order Now||Configure your project||Configure your project|