Ready-to-use, fast, sensitive, and safe Coomassie® G-250 stain

SimplyBlue™ SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie® G-250 stain for visualizing protein bands on polyacrylamide gels. This stain eliminates extensive solution preparation time and expenditure, is completely non-hazardous, and does not require acid/alcohol fixatives or destains.

Step 1
Rinse gel with three 5-minute washes with water

Step 2
Stain with SimplyBlue™ SafeStain

Step 3
Analyze gel directly in stain

Staining with SimplyBlue™ SafeStain

Fast and easy staining protocol. The SimplyBlue™ SafeStain protocol is easy to perform and can be completed in less than 3 hours (Figure 1). Bands begin to develop immediately in the stain. Destaining is not required, but may be performed to achieve maximum sensitivity and crystal-clear background. The SimplyBlue™ microwave procedure will yield results in just 12 minutes.

Sensitive staining results. SimplyBlue™ SafeStain combines a unique colloidal Coomassie® G-250 staining solution with an optimized staining protocol to guarantee nanogram-level sensitivity. 1 µg of protein can be detected after just 5 minutes in the stain. With additional water-based destaining, as little as 7 ng of protein (reduced BSA) can be detected (Figure 2).

Safe, non-hazardous, staining. Unlike traditional R-250 and some G-250 Coomassie® stains, SimplyBlue™ SafeStain does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment. In addition, SimplyBlue™ SafeStain has been independently tested for safety and confirmed to be non-hazardous, non-toxic, non-flammable, and non-corrosive. Therefore, in most cases, it can be disposed of down the drain.**

Lane 1: 6 µg protein lysate
Lane 2: 1 µg rabbit IgG
Lane 3: 1 µg reduced BSA
Lane 4: 5 µg E. coli lysate
Lane 5: 20 ng reduced BSA
Lane 6: 10 ng reduced BSA
Lane 7: 7 ng reduced BSA
Lane 8: 3 ng reduced BSA
Lane 9: 10 µl Mark12™ Standard
Lane 10: 5 µl Mark12™ Standard

Figure 2. Sensitive staining results with SimplyBlue™ SafeStain.

Samples were separated on a NuPAGE® Novex® 4-12% Bis-Tris gel, stained with SimplyBlue™ SafeStain for 1 hour, then destained overnight with the recommended water-based destain.

Contents & storage
SimplyBlue™ SafeStain is supplied as a 1X ready-to-use staining reagent. Store at room temperature. The SimplyBlue™ staining solution is guaranteed to be stable for 6 months when properly stored. There are no HazMat charges associated with this product.

* Sufficient reagent is supplied to stain 50 mini gels.
**SimplyBlue™ SafeStain has been extensively tested for safety. Ogden Environmental and Energy Services Co., Inc. San Diego, CA U.S.A, has independently confirmed these tests. For disposal requirements, check the MSDS and your local authorities for special requirements in your area.

Coomassie® is a registered trademark of Imperial Industries, PLC.