The first step in protein analysis is sample separation. Protein gel electrophoresis is the most common technique used to separate proteins from cell and tissue extracts, blood or other biological sources. Traditionally, protein gels had to be poured just prior to the electrophoretic run and the steps involved in their creation could be time-consuming and could also introduce artifacts in the experiment because of large variations in the gel composition.

Migration patterns of protein standards on NuPAGE® Novex Gels   Figure 1. Migration patterns of Mark12™ Unstained Standard on NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, and Bolt™ Gels. For optimal results, protein bands should migrate within the yellow shaded areas.

Which protein gel is right for you?

Pour-your-own essentials;
preassembled, leak-free empty cassettes
Pre-cast convenience with Laemmli chemistry Highest resolution, longest shelf-life and western optimized gels Highest resolution and long shelf-life
Order now Order now Order now Order now
  photo of gel cassette
Gel casting accessories
photo of Novex gel product
Novex® Tris-Glycine Gels
photo of Bolt gel product
Bolt® Gels
photo of NuPAGE gel product
NuPAGE® Gels

Citations in the last 10 years N/A >5,000 N/A (new product) >20,000
Protein resolution & integrity, lot consistency & band quality Low Medium High High
Average run time 90 min 90 min 35 min 35 min
Well format Standard Standard New high-capacity wedge design Standard
Gel dimensions Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick Mini: 8x8.3 cm, 1 mm thick Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick
Shelf life N/A 1–2 months >16 months 8 months (Tris-Acetate) 16 months (Bis-Tris)
Pack sizes N/A 5 & 10 packs 10 packs 2 & 10 packs

Upgrade your Bolt® Mini Gel Tank!

Upgrade your Bolt® Mini Gel Tank now to run NuPAGE®, Novex® and Bolt® gels in the same tank.

Specialized protein separation gels

We also offer a variety of gels for specialty applications including the following:

  • High throughput
  • Native protein analysis
  • Low MW protein separation
  • Protease separation
  • 2D Electrophoresis

View all specialized protein separation gels

Which protein gel is right for you?

Pour your own essentials;
Preassembled, leak-free empty cassettes
Pre-cast convenience with Laemmli chemistry Highest resolution, longest shelf-life and western optimized gels Highest resolution and long shelf-life
Order now Order now Order now Order now
 
Gel casting accessories

Novex® Tris-Glycine Express Kit

Bolt™ Gels

NuPAGE® Gels

Citations in the last 10 years N/A >5,000 N/A (new product) >20,000
Protein resolution & integrity, lot consistency & band quality Low Medium High High
Average run time 90 min 90 min 35 min 35 min
Well format Standard Standard New high capacity wedge design Standard
Gel dimensions Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick Mini: 8x8.3 cm, 1 mm thick Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick
Shelf life N/A 1 - 2 months >16 months 8 months (Tris-Acetate) 16 months (Bis-Tris)
Pack sizes N/A 5 & 10 packs 10 packs 2 & 10 packs
Price / gel (US list price) N/A $7.80 $10.30* $13.17

*Introductory pricing