The first step in protein analysis is sample separation. Protein gel electrophoresis is the most common technique used to separate proteins from cell and tissue extracts, blood or other biological sources. Traditionally, protein gels had to be poured just prior to the electrophoretic run and the steps involved in their creation could be time-consuming and could also introduce artifacts in the experiment because of large variations in the gel composition.
- Using gels from another company? Use our product selection guide to find the Life Technologies™ equivalent!
|Figure 1. Migration patterns of Mark12™ Unstained Standard on NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, and Bolt™ Gels. For optimal results, protein bands should migrate within the yellow shaded areas.|
Which protein gel is right for you?
| Pour-your-own essentials;
preassembled, leak-free empty cassettes
|Pre-cast convenience with Laemmli chemistry||Highest resolution, longest shelf-life and western optimized gels||Highest resolution and long shelf-life|
|Order now||Order now||Order now||Order now|
Gel casting accessories
Novex® Tris-Glycine Gels
|Citations in the last 10 years||N/A||>5,000||N/A (new product)||>20,000|
|Protein resolution & integrity, lot consistency & band quality||Low||Medium||High||High|
|Average run time||90 min||90 min||35 min||35 min|
|Well format||Standard||Standard||New high-capacity wedge design||Standard|
|Gel dimensions||Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick||Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick||Mini: 8x8.3 cm, 1 mm thick||Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick|
|Shelf life||N/A||1–2 months||>16 months||8 months (Tris-Acetate) 16 months (Bis-Tris)|
|Pack sizes||N/A||5 & 10 packs||10 packs||2 & 10 packs|
Upgrade your Bolt® Mini Gel Tank!
Upgrade your Bolt® Mini Gel Tank now to run NuPAGE®, Novex® and Bolt® gels in the same tank.
Specialized protein separation gels
We also offer a variety of gels for specialty applications including the following:
- High throughput
- Native protein analysis
- Low MW protein separation
- Protease separation
- 2D Electrophoresis
View all specialized protein separation gels