Recommended well loading volumes & sample loads

Table 1. Recommended well loading volumes and sample loads for mini gels

                   Number of wells/well type
  1 5 9 10 12 15 17 IPG 2D
Maximum loading volume/well Gel thickness  
1.0 mm 700 µL 60 µL 28 µL 25 µL 20 µL 15 µL 15 µL 7 cm IPG Strip 400 µL
1.5 mm n/a n/a n/a 37 µL n/a 25 µL n/a n/a 600 µL
Maximum recommended sample load for optimal resolution++ Sample type  
Protein* 12 µg 2 µg 0.5 µg 0.5 µg 0.5 µg 0.5 µg 0.5 µg n/a 12 µg
DNA** 2.4 µg 400 ng 100 ng 100 ng 100 ng 100 ng n/a n/a 2.0 µg
++NuPAGE® gels have a higher protein load capacity than Tris-Glycine or Tricine gels.  *Coomassie® stained protein; **Ethidium bromide stained DNA

Table 2. Recommended well loading volumes and sample loads for midi gels

                   Number of wells
  12 + 2** 20 26**
Maximum load volume/well* 45 µL sample
+ 15 µL standards
25 µL 15 µL
Maximum protein load/band*** 0.7 µg 0.5 µg 0.35 µg
Maximum protein load/band**** 1.4 ng 1 ng 0.7 ng
*These volumes represent approximately 60% of the actual maximum volume of the wells. ** Compatible with standard multichannel pipette; *** Coomassie® stained protein; ****Silver stained protein