Whether you require high-throughput results or a low-cost, effective means for 2D electrophoresis - or both - the ZOOM® IPGRunner™ is your system of choice.

Analyzing complex protein samples using traditional two-dimensional (2D) electrophoresis methods is tedious and time-consuming. Now you can obtain high-resolution results quickly and easily with the oil-free ZOOM® IPGRunner™ System. The mini-gel format is easy to handle and allows you to focus on the separation range you want. You'll get the separation you need, in a fraction of the time, without the hassle.

  • Separate proteins in as little as 3 hours
  • Unique design eliminates messy mineral oil
  • Easy-to-handle IPG Strips
  • Self-contained cassettes simplify rehydration and focusing

Ordering Information

Sku Name Size Price Qty
ZM0001 ZOOM® IPGRunner™ Mini-cell 1 system USD 864.00
ZM0002 ZOOM® IPGRunner™ Combo Kit 1 kit USD 1,148.00
ZM0003 ZOOM® IPGRunner™ Cassette 10 cassettes USD 275.00
ZM0007 ZOOM® Equilibration Trays 10⁄pack USD 51.00
ZM0008 ZOOM® IPGRunner™ Cassettes and Trays 10 trays USD 301.00
ZM0011 ZOOM® IPG Strips - pH 3-10NL (non-linear) 12 strips USD 121.00
ZM0012 ZOOM® IPG Strip pH 4-7 12 strips USD 121.00
ZM0013 ZOOM® IPG Strip pH 6-10 12 strips USD 121.00
ZM0014 ZOOM® IPG Strip pH 4.5-5.5 12 strips USD 121.00
ZM0015 ZOOM® IPG Strip pH 5.3-6.3 12 strips USD 121.00
ZM0016 ZOOM® IPG Strips pH 6.1-7.1 12 strips USD 121.00
NP0330BOX NuPAGE® Novex® 4-12% Bis-Tris ZOOM® Protein Gels, 1.0 mm, IPG well 1 box USD 157.00
EC60261BOX Novex® 4-20% Tris-Glycine ZOOM® Protein Gels, 1.0 mm, IPG well 1 box USD 150.80
ZM0021 ZOOM® Carrier Ampholytes pH 3-10 10 mL USD 233.00
ZM0022 ZOOM® Carrier Ampholytes pH 4-7 10 mL USD 251.00
ZM0023 ZOOM® Carrier Ampholytes pH 6-9 10 mL USD 257.00
ZM0024 ZOOM® Carrier Ampholytes pH 9-11 10 mL USD 251.00
ZM0025 ZOOM® Carrier Ampholytes 4-6, 10mL 10 mL USD 251.00
NP0008 NuPAGE® LDS Sample Buffer (4X) 250 mL USD 120.00
NP0009 NuPAGE® Sample Reducing Agent (10X) 10 mL USD 55.75
LC6070 SilverQuest™ Silver Staining Kit 1 kit USD 262.00
LC6060 SimplyBlue™ SafeStain 1 L USD 134.00

1D/2D Electrophoresis - ZOOM® IPGRunner™

First Dimension (IEF):

The ZOOM® IPGRunner™ System is an oil-free platform for focusing of up to 12 samples, in as little as three hours.

The 7 cm ZOOM® Strips are immobilized pH gradient (IPG) gels available in wide pH range formats (3 – 10, 4 – 7, and 6 – 10) for broader analysis, and in one-unit narrow pH range strips (4.5 – 5.5, 5.3 – 6.3, and 6.1 – 7.1) for expanded protein separation within a specific region of interest.


The ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups, that sort at their relative position based upon their isoelectric field, setting up a pH gradient. Carrier ampholytes help to stabilize the pH gradient and current in IPG strips and aid in protein solubility, resulting in reproducible IEF resolution.

Second Dimension (SDS-PAGE):

Following isoelectric focusing, the ZOOM® Strips load easily into the NuPAGE® and Novex® ZOOM® Gels, for 2D separation of proteins by molecular weight.

The separated proteins can then be visualized with any of a number of stains, blotted to membranes, or digested and extracted for identification by mass spectrometry.

ZOOM® 2D Protein Solubilizers are ready-to-use detergent chaotrope blends that enhance solubilization of the most difficult proteins, including hydrophobic and membrane proteins, and improve resolution and detection of novel proteins by 2DE. ZOOM® 2D Protein Solubilizers:

  • Save time and eliminate preparation errors
  • Provide reproducible results
  • Retain protein solubility throughout 1D separation

ZOOM® Proteomic Grade Reagents

Invitrogen also offers a comprehensive line of ZOOM® proteomic grade reagents, including Urea, Thiourea, CHAPS, carrier ampholytes pH 3 – 10, focusing buffers, anode buffers, and cathode buffers to ensure optimal conditions for sample preparation.

With the ZOOM® IPGRunner™ System, complete 2D electrophoresis is possible in less than 5 hours from sample to image.

Fractionator Combo Kit

 

Fractionation Products

 

   

 

1D/2DE Products

References

  1. Boldt, A, Fortunato, D, Conti, A, Petersen, A, Ballmer-Weber, B, Lepp, U, Reese, G, and Becker, W-M “Analysis of the composition of an immunoglobulin E reactive high molecular weight protein complex of peanut extract containing Ara h 1 and Ara h 3/4” Proteomics 2005 Feb;5(3):675-86.
  2. Gonzalez, S, Prives, C, and Cordon-Cardo, C “p73a Regulation by Chk1 in Response to DNA Damage” Mol. Cell. Biol. 2003 Nov;23(22):8161-71.
  3. Tetlow, IJ, Wait, R, Lu, Z, Akkasaeng, R, Bowsher, CG, Esposito, S, Kosar-Hashemi, B, Morell, MK, and Emes, MJ “Protein Phosphorylation in Amyloplasts Regulates Starch Branching Enzyme Activity and Protein-Protein Interactions” Plant Cell 2004 Mar;16:694-708.
  4. Tawde, PD “Allergenic Cross-reactivity between Cashew and Pistachio Nuts” 2004 Masters Thesis, Dept. Biol. Sci. The Florida State University
  5. Zancai P, Dal Col J, Piccinin S, Guidoboni M, Cariati R, Rizzo S, Boiocchi M, Maestro R, Dolcetti R. “Retinoic acid stabilizes p27(Kip1) in EBV-immortalized lymphoblastoid B cell lines through enhanced proteasome-dependent degradation of the p45(Skp2) and Cks1 proteins” Oncogene. 2005 Feb 14; [Epub ahead of print]