The ZOOM® IEF Fractionator offers a fast, reliable method to reduce sample complexity, enrich low abundance proteins, and increase the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM® IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two dimensional gel electrophoresis (2DE), one dimensional gel electrophoresis (1DE), or two dimensional liquid chromatography/mass spectrometry (2D LC/MS).

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The ZOOM® IEF Fractionator combo kit

Components of this kit include:

The ZOOM® IEF Fractionator—built for safety, reliability, and durability. It includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid.

ZOOM® Disks—precast polyacrylamide gels that eliminate the need for manual preparation and minimize any chance of cross contamination. These immobilized buffered disks are pre-labeled, disposable, and designed for single use, ensuring consistent and reproducible fractionation. They resolve samples into five equal fractions (using six disks of specific pH) in the pH range from 3–10.

ZOOM® Reagents—proteomic grade reagents, including urea, thiourea, CHAPS, and ampholytes, that ensure optimal conditions for sample preparation.

Contents and storage:

The ZOOM® Fractionator combo kit includes the ZOOM® IEF Fractionator; one pack ZOOM® Disks of each pH; and all ZOOM® Reagents (urea, thiourea, CHAPS, carrier ampholytes pH 3–10, anode buffers, and cathode buffers). ZOOM® Disks, Carrier Ampholytes, and Buffers are stored at 4°C.

   Zoom IEF Fractionator Combo Kit

Downstream processing by 2D gel electrophoresis

Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM® Strips on the ZOOM® IPGRunner™ System. Follow with second dimension analysis using neutral pH NuPAGE® ZOOM® Gels.

Technical resources

Manual
Quick Reference Card

References

  1. Zuo, X. and Speicher, D.W. (2000) Anal. Biochem. 284: 266-278.
  2. Zuo, et. al. (2001) Electrophoresis 22: 1603-1615.
  3. Zuo, X. and Speicher, D.W. (2002) Proteomics: 2: 58-68.
  4. Zuo, X., Hembach, P., Echan, L., and Speicher, D.W. (2002) Journal of Chromatography B 782: 253-265.
  5. Ali-Khan, N., Zuo, X., and Speicher, D.W. (2002) Current Protocols in Protein Science 22.1: 1-19.
  6. Zuo, X. and Speicher, D.W. (2000) Methods in Molecular Biology (Humana Press, P. Cutler, ed.) In press.
  7. Zuo, X. and Speicher D.W. 2004. Microscale solution isoelectrofocusing: A sample prefractionation method for comprehensive proteome analysis. In: Methods in Molecular Biology: Protein Purification Protocols. Humana Press, Totowa, NJ (P. Cutler, ed.), pp.361-375.
  8. Speicher, D.W. 2004. Overview of proteome analysis. In: Proteome Analysis: Interpreting the Genome (D. Speicher, ed.). Elsevier, The Netherlands, pp. 1-18.
  9. Zuo, X., Lee, K., Speicher, D.W. 2004. Electrophoretic prefractionation for comprehensive analysis of proteomes. In: Proteome Analysis: Interpreting the Genome (D. Speicher, ed.). Elsevier, The Netherlands, pp. 93-118.
  10. Speicher, D.W., Lee, K., Tang, H.-Y., Echan, L., Ali-Khan, N., Zuo, X., and Hembach, P. 2004. Current challenges in proteomics: Mining low abundance proteins and expanding protein profiling capacities. In: Advances in Mass Spectrometry, Vol. 16 (Ashcroft, A.E., Brenton, G, and Monaghan, J.J., ed.) pp. 37-57.
  11. Zuo, X., Lee, K., and Speicher, D.W. 2004. Fractionation of complex proteomes by microscale solution isoelectrofocusing using ZOOM IEF fractionators to improve protein profiling. In: The Proteome Handbook (Walker, J.M., ed.) In press.

These products may be covered by one or more Limited Use Label Licenses. By the use of these products you accept the terms and conditions of all applicable Limited Use label Licenses.

IPGRunner™, SimplyBlue™, and XCell SureLock™, are trademarks of Invitrogen Corporation. Novex®, NuPAGE®, and ZOOM® are registered trademarks of Invitrogen Corporation.

The ZOOM® IEF Fractionator was in part developed pursuant to a license from The Wistar Institute of Anatomy and Biology, located in Philadelphia, Pennsylvania. Established in 1892, The Wistar Institute was the first independent medical research facility in the United States.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.