Novex® Tris-Glycine gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis with minor modifications for maximum performance in the pre-cast format. Unlike traditional Laemmli gels with a stacking gel pH of 6.8 and separating gel pH of 8.8, Novex® Tris-Glycine gels have a pH of 8.65 for both regions. Novex® Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Novex® Tris-Glycine gels are:

See the Tris-Glycine Gel Migration Charts

Choose the right Novex® Tris-Glycine gel

New optimized protocols!

Check out these completely revamped protocols for Novex® Tris-Glycine gels:

Getting started with Novex® Tris-Glycine gels

Get optimal separation of your proteins by choosing the right combination of gel and running buffer. Available concentrations, sizes, and buffer systems are given below. 

Buffering systems
Novex® Tris-Glycine gels do not contain SDS and can be used to run your proteins in native form or in denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.

Polyacrylamide concentrations
Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. Each box contains 10 gels.

To determine what type of gel should be used to separate your proteins, please refer to Figure 1 for the protein migration patterns.

Gel sizes
Novex® Tris-Glycine gels  are available in two different  sizes: mini gels (8 cm x 8 cm) and midi gels (8 cm x 13 cm).  Both mini gels and midi gels are available with 1.0 mm gel thickness but the 1.5 mm gel thickness is available only with the mini gel format. Novex® Tris-Glycine gels come in multiple-well formats, ranging from 1 well to 26 wells. For loading volumes, see Recommended Well Loading Volumes & Sample Loads .

 
Novex® Tris-Glycine Gel migration patterns

Figure 1. Migration patterns of Invitrogen protein molecular weight standards on Novex® Tris-Glycine Gels. For optimal results, protein bands should migrate within the yellow shaded areas.

The Tris-Glycine discontinuous buffer systems utilizes three ions:

  • Chloride (–) from the gel buffer serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Tris+ and Cl– (pH 8.65).
  • Glycine (–) is the primary anion in the running buffer and serves as a trailing ion. Glycine is partially negatively charged and trails behind the highly charged chloride ions in the charged environment. The running buffer ions are Tris+, Gly–, and dodecylsulfate– (pH 8.3).
  • Tris base (+) is the common ion present in the gel buffer and running buffer. During electrophoresis, the gel and buffer ions in the Tris-Glycine system form an operating pH of 9.5 in the separation region of the gel.

Gel storage and shelf-life

For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase. 16% and 18% Novex® Tris-Glycine gels should be used within 4 weeks of purchase.