Novex® Tris-Glycine Gels
Novex® Tris-Glycine gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis with minor modifications for maximum performance in the pre-cast format. Unlike traditional Laemmli gels with a stacking gel pH of 6.8 and separating gel pH of 8.8, Novex® Tris-Glycine gels have a pH of 8.65 for both regions. Novex® Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.
Novex® Tris-Glycine gels are:
- Individually packaged for convenience
- Compatible with most protein standards for accurate size determination
- Flexible for use with native and denatured protein samples
- Using gels from another company? Use our product selection guide to find the Life equivalent!
Choose the right Novex® Tris-Glycine gel
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Getting started with Novex® Tris-Glycine gels
Get optimal separation of your proteins by choosing the right combination of gel and running buffer. Available concentrations, sizes, and buffer systems are given below.
Novex® Tris-Glycine gels do not contain SDS and can be used to run your proteins in native form or in denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.
Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. Each box contains 10 gels.
To determine what type of gel should be used to separate your proteins, please refer to Figure 1 for the protein migration patterns.
Figure 1. Migration patterns of Invitrogen protein molecular weight standards on Novex® Tris-Glycine Gels. For optimal results, protein bands should migrate within the yellow shaded areas.
The Tris-Glycine discontinuous buffer systems utilizes three ions:
- Chloride (–) from the gel buffer serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Tris+ and Cl– (pH 8.65).
- Glycine (–) is the primary anion in the running buffer and serves as a trailing ion. Glycine is partially negatively charged and trails behind the highly charged chloride ions in the charged environment. The running buffer ions are Tris+, Gly–, and dodecylsulfate– (pH 8.3).
- Tris base (+) is the common ion present in the gel buffer and running buffer. During electrophoresis, the gel and buffer ions in the Tris-Glycine system form an operating pH of 9.5 in the separation region of the gel.
Gel storage and shelf-life
For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase. 16% and 18% Novex® Tris-Glycine gels should be used within 4 weeks of purchase.