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Novex® TG Express Protein Gel Electrophoresis Kits include 5 gels and standard 

Novex® Tris-Glycine Express Protein Gel Electrophoresis Kits now make it convenient and easy to perform fast, reproducible electrophoresis. By combining one of our most popular standards with our traditional Novex® Tris-Glycine precast gel, all reagent preparation is done for you so you can focus on results.  The kits include 5 individually wrapped precast gels with matched SeeBlue® standard. Each kit is prepared for 5 gel runs.

Getting started with Novex® TG Express Electrophoresis Kits

Each kit comes with a preselected SeeBlue® standard. Simply select the appropriate buffer for your run.

Buffering systems

Novex® Tris-Glycine gels do not contain SDS and can be used to run proteins in native or denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.

The Tris-Glycine discontinuous buffer systems utilize three ions:

  • Chloride (–) from the gel buffer serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Tris+ and Cl– (pH 8.65).
  • Glycine (–) is the primary anion in the running buffer and serves as a trailing ion. Glycine is partially negatively charged and trails behind the highly charged chloride ions in the charged environment. The running buffer ions are Tris+, Gly–, and dodecylsulfate– (pH 8.3).
  • Tris Base (+) is the common ion present in the gel buffer and running buffer. During electrophoresis, the gel and buffer ions in the Tris-Glycine system form an operating pH of 9.5 in the separation region of the gel.

Gel storage and shelf-life

For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase.

Recommended well-loading volumes and sample loads for mini gels

  10 12 15
Maximum loading volume/well 1.0 mm 25 µL 20 µL 15 µL
  1.5 mm  37 µL  n/a 25 µL
Maximum recommended sample load for optimal resolution++  Protein*  0.5 µg  0.5 µg 0.5 µg