Novex® Tris-Glycine Express Protein Gel Electrophoresis Kits
Novex® TG Express Protein Gel Electrophoresis Kits include 5 gels and standard
Novex® Tris-Glycine Express Protein Gel Electrophoresis Kits now make it convenient and easy to perform fast, reproducible electrophoresis. By combining one of our most popular standards with our traditional Novex® Tris-Glycine precast gel, all reagent preparation is done for you so you can focus on results. The kits include 5 individually wrapped precast gels with matched SeeBlue® standard. Each kit is prepared for 5 gel runs.
Getting started with Novex® TG Express Electrophoresis Kits
Each kit comes with a preselected SeeBlue® standard. Simply select the appropriate buffer for your run.
Novex® Tris-Glycine gels do not contain SDS and can be used to run proteins in native or denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.
The Tris-Glycine discontinuous buffer systems utilize three ions:
- Chloride (–) from the gel buffer serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Tris+ and Cl– (pH 8.65).
- Glycine (–) is the primary anion in the running buffer and serves as a trailing ion. Glycine is partially negatively charged and trails behind the highly charged chloride ions in the charged environment. The running buffer ions are Tris+, Gly–, and dodecylsulfate– (pH 8.3).
- Tris Base (+) is the common ion present in the gel buffer and running buffer. During electrophoresis, the gel and buffer ions in the Tris-Glycine system form an operating pH of 9.5 in the separation region of the gel.
Gel storage and shelf-life
For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase.
Recommended well-loading volumes and sample loads for mini gels
|Maximum loading volume/well||1.0 mm||25 µL||20 µL||15 µL|
|1.5 mm||37 µL||n/a||25 µL|
|Maximum recommended sample load for optimal resolution++||Protein*||0.5 µg||0.5 µg||0.5 µg|