Dynabeads® coated with secondary antibodies
For simple, robust, and rapid small-scale purification of immunoglobulins and subsequent isolation of target protein, (immunoprecipitation) you can also use Dynabeads® Protein A and Dynabeads® Protein G.
- Ideal for isolation of small components such as proteins and peptides
- Flexible technology used for a wide variety of applications
- Convenient and simple to use
- Recommended for use in sandwich immunoassays
- Superior binding kinetics (Figure 1)
Figure 1. Dynabeads® binding kinetics are superior to traditional microtitre plates. The graph shows % binding of tumor necrosis factor to immobilised antibody as a function of time. (Courtesy of N-B Liabakk, Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Norway.)
Direct or Indirect Method
- The Direct Method: The primary antibody is bound to the Dynabeads® , the Ab-bound beads are then incubated with the sample.The direct method is well suited when the target protein is abundant. This allows you to make up a stock of beads with bound antibody, and requires less primary antibody.
- The Indirect Method: The primary antibody is first incubated with the sample to form an Ab-antigen complex. This complex is then captured by adding Dynabeads® to the sample, followed by magnetic separation. The indirect method is well suited when the antibody has a poor affinity for the target or where the target is low abundant. Thisgives minimal co-incubation of Dynabeads® with the sample, hence allows optimal control of background binding.