This is a list of selected references citing the use of Dynabeads® for different protein isolation applications.

Isolations of active enzymes

  • Barnes K. et al. Immunoseparation of membrane peptidases from pig lung membranes using magnetic beads. Biochem. Soc.Trans. 1994;22:451S.
  • Bones AM. et al. Fate of myrosin cells: Characterization of monoclonal antibodies against myrosinase. J. Exp. Botany 1991;42:1541-49.
  • Gross SD. et al. A casein kinase I isoform is required for proper cell cycle progression in the fertilized mouse oocyte. J. Cell. Sc.1997;110:3083-90.
  • Jost J-P. et al. Mechanisms of DNA demethylation in chick embryos. J. Biol. Chem. 1995;270:9734-39.
  • Laser M. et al. Differential activation of p70 and p85 kinase isoforms during cardiac hypertrophy in the adult mammal. J. Biol. Chem.1998;273(38):24610-19.
  • Liao S-M. et al. A kinase-cyclin pair in the RNA polymerase II holoenzyme. Nature 1995;374:193-96.

Immunopurifications of proteins

  • Chakraborty A. et al. Identification of a novel Stat3 recruitment and activation motif within the granulocyte colony-stimulating factor receptor. Blood 1999;93:15-24.
  • Karlsson GB and Platt FM. Analysis and isolation of human transferrin receptor using the OKT-9 monoclonal antibody covalently crosslinked to magnetic beads. Anal. Biochem. 1991;199:219-22.
  • Knutson VP. et al. Insulin resistance is mediated by a proteolytic fragment of the insulin receptor. J. Biol. Chem. 1995;270(42):24972-81.
  • McKay SJ and Cooke H. hnRNP A2/B1 binds specifically to single stranded vertebrate telomeric repeat TTAGGGn. Nucleic Acids Res. 1992;20(24):6461-64.
  • Rabouille C. et al. Syntaxin 5 is a common component of the NSF- and p97- mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro. Cell 1998;92:603-10.
  • Tuscano J. et al. Engagement of the adhesion receptor CD22 triggers a potent stimulatory signal for B cells and blocking CD22/CD22L interactions impairs T-cell proliferation. Blood 1996;87:4723-30.

Isolations via coupled peptides

  • Lauer JL. et al. Effect of ligand conformation on melanoma cell alpha3 beta1 integrin-mediated signal transduction events: Implications for a collagen structural modulation mechanism of tumor cell invasion. Biochemistry 1998;37:5279-87.
  • Lauer JL. et al. Inhibition of melanoma cell binding to type IV collagen by analogs of cell adhesion regulators. J. Med. Chem. 1997;40:3077-84.
  • Re F. et al. Inhibition of anchorage-dependent cell spreading triggers apoptosis in cultured human endothelial cells. J. Cell Biol. 1994;127:537-46.
  • Zanetti A. et al. Clustering of vitronectin and RGD peptides on microspheres leads to engagement of integrins on the luminal aspect of endothelial cell membrane. Blood 1994;84(4):1116-23.
  • Traverso G et al. Multicolor in vitro translation. Nat. Biotechnol. (2003) 21(9):1093-1097

Isolations via coupled carbohydrates

  • Higgins DA and Ko WKW. Duck lymphocytes. VII. Selection of subpopulations using lectin-coated magnetic beads. Vet. Immunol. Immunopathol. 1995;44:181-95.
  • Rye PD, Bovin NV. Selection of carbohydrate-binding cell phenotypes using oligosaccharide-coated magnetic particles. Glycobiology 1997;7:179-82.
  • Rye PD. Sweet and Sticky: Carbohydrate-coated magnetic beads. BioTechnology 1996;14:155-57.
  • Weersink AJL. et al. Human granulocytes express a 55-kDa lipopolysaccharide-binding protein on the cell surface that is identical to the bactericidal/permeability- increasing protein. J. Immunol. 1993;150:253-63.

Phage display

  • Demaris S. et al. A Strategy for the isolation of catalytic activities from repertoires of enzymes displayed on a phage, J. Mol. Biol. 1999;286:617-33.
  • McConnell SJ. Biopanning Phage Display libraries using magnetic beads vs.polystyrene plates. BioTechniques. 1999; 26:208-14.
  • Nord K. Binding proteins selected from combinatorial libraries of an a-helical bacterial receptor domain. Nat. Biotechnol. 1997;15:772-77.
  • Russell SJ. Retroviral vectors displaying functional antibody fragments. Nucleic Acids Res. 1993;21(5):1081-85.
  • Sawyer C. et al. Methodology for selection of human antibodies to membrane proteins from a phage-display library. J. Immunol. Meth. 1997; 204: 193-203.
  • Wang L. et al. Cloning of anti-Gal Fabs from combinatorial phage display libraries: Structural analysis and comparison of Fab expression in pComb3H and pComb8 phage. Mol. Immunol. 1999;34:609-18.

Bio- and immunoassays

  • Cudjoe KS. et al. Immunomagnetic separation of Salmonella from foods and their detection using immunomagnetic particle (IMP)-ELISA. Int. J. Food Microbiol. 1995;27:11-25.
  • Ginis I. et al. Characterization of a hypoxia-responsive adhesion molecule for leukocytes on human endothelial cells. J. Immunol. 1995;155:802-10.
  • Haukanes B-I and Kvam C. Application of magnetic beads in bioassays. BioTechnology 1993;11(1):60-63.
  • Leivestad T. et al. A simple and sensitive bioassay for the detection of IL-2 activity. J. Immunol. Meth. 1988; 114:95-99.
  • Liabakk N-B. et al. A rapid and sensitive immunoassay for tumor necrosis factor using magnetic monodisperse polymer particles. J. Immunol. Meth. 1990;134:253-59.
  • Wognum AW. et al. A specific in vitro bioassay for measuring erythropoietin levels in human serum and plasma. Blood 1990;76(7):1323-29. Isolation of eukaryotic cells
  • Barth RJ. et al. Unique murine tumor-associated antigens identified by tumor infiltrating lymphocytes. J. Immunol. 1990;144(4):1531-37.
  • Bertoncello I. et al. An improved negative immunomagnetic selection strategy for the purification of primitive hemopoietic cells from normal bone marrow. Exp. Hematol. 1991;19(2).
  • Gaur NK and Klotz SA. Expression, cloning and characterization of a Candida albicans gene, ALA, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins. Infection and Immunity 1997;65:5289-94.
  • Hewett PW and Murray JC. Immunomagnetic purification of human microvessel endothelial cells using Dynabeads coated with monoclonal antibodies to PECAM-1. Eur. J. Cell Biol. 1993;62:451-54.
  • Levine BL. et al. Antiviral effect and ex vivo CD4+ T cell proliferation in HIV-positive patients as a result of CD28 costimulation. Science 1996;272:1939-43.
  • Nandigala P. et al. Immunomagnetic isolation of islets from the rat pancreas. Biotechnol.Prog. 1997;13:844-48.
  • Rye PD. et al. A novel approach for the isolation and propagation of tumor cells. Am. J. Pathol. 1997;150:99-105.
  • Ushijima H. et al. Removal of HIV antigens and HIV-infected cells in vitro using immunomagnetic beads. J. Virol. Methods 1990;29:23-32.

Isolation of bacteria

  • Bird JA. et al. Rapid salmonella detection by a combination of conductance and immunological techniques. Rapid Microbiological Methods for Foods,Beverages and Pharmaceuticals 1989;165-83.
  • Blake MR and Weimer BC. Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples. Appl. Environ. Microbiol. 1997;63:1643-46.
  • Islam D and Lindberg AA. Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction. J. Clin. Microbiol. 1992;30(11):2801-06.
  • Lamoureux M. et al. Detection of Campylobacter jejuni in food and poultry viscera using immunomagnetic separation and microtitre hybridization. J. Appl. Microbiol. 1997;83:641-51.
  • Tomoyasu T. Development of the immunomagnetic enrichment method selective for Vibrio parahaemolyticus serotype K and its application to food poisoning study. Appl. Environ. Microbiol. 1992; 58(8):2679-82.