Dynabeads® act as a solid-phase with rapid liquid-phase kinetics, perfect for high-quality immunoprecipitation (IP) procedures.  When placed on a magnet, the beads will quickly and gently migrate to the tube wall, allowing for quick buffer exchange and washing.

IP Selection guide

Immunoprecipitation (IP) & Co-Immunoprecipitation (Co-IP)

Bead
Surface
non-specific
Binding
Antibody (Ab) Coupling Characteristics Dynabeads
Surface
Coating
Select Your
Product
Format
Species
Compatibility
for source
antibodies
Are antibodies
covalently
coupled to
bead surface?
Are
purified
antibodies
required?
Coupling
Reaction
Time
Lowsee table
below
no*no10 minProtein ABeads only
IP KIT
Protein G
(top seller)
Beads Only
IP KIT
LowMousenono30 minSheep α-MouseBeads Only
Rabbitnono30 minSheep α-RabbitBeads Only
Ultra LowAnyYesnoovernightEpoxy
(top seller)
Beads Only
Antibody Coupling KIT
Co-IP KIT

* Antibodies can be covalently linked onto Protein A & G Dynabeads using commercially available crosslinkers.

Benefits of crosslinking include covalently locking antibodies onto the bead surface such that they do not co-elute with immunoprecipitated target proteins during the final elution step.  This will enable you to run a gel without having the antibody heavy and light chains co-migrating with immunoprecipitated proteins.  Anytime that you crosslink antibodies using crosslinking reagents there is a significantly increased risk of dramatically reducing the yield of the immunoprecipitation while simultaneously decreasing the antibodies specificity to its target(s).  The end result of crosslinking will likely be increased non-specific antibody-ligand binding and reduced capacity.

For covalent antibody coupling to Dynabeads, we therefore recommend Dynabeads® Tosylactivated and Dynabeads® M-270 Epoxy (now available with all buffers required for antibody coupling as the Dynabeads® Antibody Coupling Kit: also linked in the table above).  These beads and kits provide all of the upsides of covalent antibody coupling to beads without any of the potential pitfalls that crosslinking can introduce.

Antibody Compatibility Table: Dynabeads® Protein A & Dynabeads® Protein G

Species Ig Subclass Protein A Protein G
HumanIgG1, IgG2, IgG4
IgG3
IgD
IgD
Fab
ScFv
+++
+

+
+
+
+++
+++


+
MouseIgG1
IgG2a, IgG2b, IgG3
IgM
+
+++
++
+++
RatIgG1
IgG2a
IgG2b
IgG2c
+


+++
++
+++
+
+++
GoatIgG1
IgG2
+
+++
+++
+++
SheepIgG1
IgG2
+
+++
+++
+++
Cow / BovineIgG1
IgG2
+
+++
+++
+++
HorseIgG(ab)
IgG(c)
IgG(T)
+
+


+++
RabbitTotal Ig++++++
DogTotal Ig++++
CatTotal Ig++++
PigTotal Ig++++
Guinea pigTotal Ig++++
ChickenTotal Ig
+++ = strong binding; ++ = medium binding; + =  weak binding; – = no binding

Protein G and protein A exhibit variation in binding strength to different Immunoglobulins (Ig).  This variation exists both between different species and between different antibody subclasses from the same species. (As an example, human IgG3 will bind strongly to protein G, but only weakly to protein A.) The table above gives an overview of binding strengths of protein G and protein A to different Ig species and subclasses. 

For Igs with weak affinity to both protein A and protein G (or for Ig subclasses not specified in the table above, we recommend the Dynabeads® Antibody Coupling Kit which is compatible with all antibody species and subclasses.