Invitrogen’s resins are suitable for purification and fractionation of proteins and antibodies using column or magnetic bead chromatography or immunoprecipitation methods. A wide array of products are available as both bulk materials as well as part of convenient kits that include purification buffers, columns and antibodies. If your recombinant protein contains the recognition sequence for enterokinase (EnterokinaseMax™), AcTEV™ or SUMO Protease, you may cleave the tag from the fusion protein using the specific protease. Find the optimum Invitrogen product for your protein in the Purification Selection Guide.

Selection Guide

 

Resin Purification
Histidine-tagged recombinant proteins through pre-charged Ni on a NTA resinNi-NTA Agarose
Ni-NTA Agarose Kit
Histidine-tagged recombinant proteins through an iminodiacetic acid ligandProBond™
ProBond Kit™
Histidine-tagged recombinant proteins using magnetic beads.Dynabeads® His-Tag Isolation & Pulldown
Proteins with Glutathione S-Transferase (GST) tailGlutathione agarose
Biotinylated ProteinsStreptavidin Agarose
Dynabeads® Streptavidin
IgG molecules from mouse, human, rabbit, and Guinea pig with Protein A isolated from Staphylocccus aureusProtein A Sepharose®
IgG purification as well as fractionation of IgG fragments, fractionation of cells and isolation of antigen-antibody complexDynabeads® Protein A
rProtein A Agarose
IgG from goat, sheep, horse, cow, rat, mouse, human, rabbit, and Guinea pigDynabeads® Protein G 
rProtein G Agarose
IgG from MouseDynabeads® M-280 Sheep anti-Mouse IgG
IgG from RabbitDynabeads® M-280 Sheep anti-Rabbit IgG
Strong affinity for IgG as well as IgA, IgE and IgMKappaLock
Mouse monoclonal IgG1 from ascitesMono Ab rec-Protein A System
Protein A immunosorbentZysorbin
Immobilization of sulfhydryl-containing compoundsRAP – Rapid Affinity Purification Kit
Removal of EKMax or other enterokinase enzymesEK-Away™ Resin
Cleavage of Fusion Peptides
Removal of affinity tags by cleaving at Glu-Asn-Leu-Tyr-Phe-Gln-Gly recognition sequenceAcTEV™ Protease
Cleavage of fusion proteins with Asp-Asp-Asp-Asp-Lys peptide sequenceEKMax™ Enterokinase
Recognition of tertiary structure of SUMOSUMO Protease

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