Antibodies Other Ligands
Figure 1.  Antibodies or other ligands can be coupled onto the beads for affinity protein capture.

A Wide Range of Products - Flexibility for Your Research

Surface-activated Dynabeads® are available in different sizes, with a hydrophobic or hydrophilic nature, and with a variety of surface chemistries (see Table 1 below). To obtain the best possible results it is important to choose a size and surface of the Dynabeads® suited for your application. The choice will depend on the nature of the ligand. In most applications several Dynabeads® products can be used.

Table 1 - Choose the right surface-activated Dynabeads® for your application

Ligand Target Dynabeads® Tosyl-
activated
Dynabeads®
Carboxylic
Acid
Dynabeads® Amine Dynabeads® Epoxy Dynabeads® Subcellular
AntibodyLow MW antigen1 or peptide 
Protein or antibody
Protein complex




Organelles

2
Phage1
Virus
Bacteria


Cells3

Antibody FragmentPhage1 or antibody
ProteinPhage or carbohydrate
Nucleic acid
PeptidePhage1 or antibody
CarbohydrateAntibody

Low MV antigen Antibody
Nucleic acids, oligonucleotide, aptamer, PNANucleic acid binding proteins1 4

DNA1, RNA1 or PCR amplicons1
4

EnymeSubstrate or target for enzyme degradation
Organic chemistry derivatization, including introduction of new functional groups



- Best product choice for listed ligand and target.
- Alternative choice for listed ligand and target.
- Can be used for listed ligand and target.
1 - Dynabeads® pre-coupled with streptavidin may be the best product choice.
2 - Dynabeads® M-450 Epoxy.
3 - Dynabeads® M-450 are recommended for isolation, stimulation, and expansion of cells.
4 - Dynabeads® Amine is modified with an appropriate bifunctional crosslinker prior to immobilization of oligonucleotides.