Life Technologies resins are suitable for purification and fractionation of proteins and antibodies using column or magnetic bead chromatography or immunoprecipitation methods. A wide array of products are available as both bulk materials as well as part of convenient kits that include purification buffers, columns and antibodies. If your recombinant protein contains the recognition sequence for enterokinase (EnterokinaseMax™), AcTEV™ or SUMO Protease, you may cleave the tag from the fusion protein using the specific protease. Find the optimum Invitrogen product for your protein in the Purification Selection Guide below.

Table 1 - Selection Guide

Application Products
Resin Purification
Histidine-tagged recombinant proteins through pre-charged Ni on a NTA resin Ni-NTA Agarose
Ni-NTA Agarose Kit
Histidine-tagged recombinant proteins through an iminodiacetic acid ligand ProBond™
ProBond Kit™
Histidine-tagged recombinant protein isolation using magnetic beads Dynabeads® His-Tag Isolation & Pulldown
Proteins with Glutathione S-Transferase (GST) tail Glutathione agarose
Mouse anti-GST-Sepharose® 4B
Biotinylated Proteins Dynabeads® Streptavidin Streptavidin Agarose
Immunoprecipitation of protein complexes Dynabeads® Co-Immunoprecipitation Kit
Covalent antibody coupling to beads Dynabeads® Antibody Coupling Kit
IgG molecules from mouse, human, rabbit, and Guinea pig with Protein A isolated from Staphylocccus aureus Protein A Sepharose®
IgG purification as well as fractionation of IgG fragments, fractionation of cells and isolation of antigen-antibody complex Dynabeads® Protein A
rProtein A Agarose
IgG from goat, sheep, horse, cow, rat, mouse, human, rabbit, and Guinea pig Dynabeads® Protein G
rProtein G Agarose
IgG from Mouse Dynabeads® M-280 Sheep anti-Mouse IgG
IgG from Rabbit Dynabeads® M-280 Sheep anti-Rabbit IgG
Strong affinity for IgG as well as IgA, IgE and IgM KappaLock
Mouse monoclonal IgG1 from ascites Mono Ab rec-Protein A System
Protein A immunosorbent Zysorbin
Immobilization of sulfhydryl-containing compounds RAP – Rapid Affinity Purification Kit
Removal of EKMax or other enterokinase enzymes EK-Away™ Resin
Cleavage of Fusion Peptides
Removal of affinity tags by cleaving at Glu-Asn-Leu-Tyr-Phe-Gln-Gly recognition sequence AcTEV™ Protease
Cleavage of fusion proteins with Asp-Asp-Asp-Asp-Lys peptide sequence EKMax™ Enterokinase
Recognition of tertiary structure of SUMO SUMO Protease

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SEPHAROSE® is a trademark of GE Healthcare
The technology used for Dynabeads® TALON® is licensed from Clontech Laboratories Inc., USA.  TALON® is a registered trademark of Clontech Laboratories Inc., USA.