• One reagent convenience for DNA or RNAi transfection
  • Simple protocol for easy optimization in a wide range of cell types
  • Overwhelmingly more publications cite Lipofectamine® 2000 Reagent

Transfect your cells with confidence

Lipofectamine® 2000 Reagent delivers DNA or siRNA with excellent transfection performance for protein expression, gene silencing, and functional assays. Superior efficiency and simple protocol for success across a broad range of cell lines (figure 1)—Lipofectamine® 2000 Reagent does it all.

Figure 1. Lipofectamine® 2000 Reagent yields high transfection efficiencies in a variety of cell lines. Representative images are shown for the conditions that produced optimal transfection. Blue cells are those that express β-lactamase and therefore have been successfully transfected with the reporter vector. Green cells are untransfected. GripTite™ 293 MSR cells untreated (A) and transfected using 0.5 μg of Lipofectamine® 2000 per well (B). HeLa cells untreated (C) and transfected using 0.25 μg of Lipofectamine® 2000 (D). MCF-7 cells untreated (E) and transfected using 0.5 μg of Lipofectamine® 2000 (F).high transfection efficiencies in a variety of cell lines. 

Lipofectamine recognition

Publications citing Lipofectamine® 2000 have skyrocketed since its launch in 1999 and continue to climb (Figure 2). Lipofectamine® 2000’s huge success can be attributed to its performance, delivering a high level of expression and transfection efficiency on a broad range of cell types. Lipofectamine® reagents are the most cited and recognized reagents available for transfection.

Lipofectamine recognition

Figure 2. Number of publications citing the use of Lipofectamine® 2000 transfection reagent since 1999.