The iBind™ Western System is an automated western processing device. It’s hands-free, reproducible, powerless and compatible with western protocols.
Allows you to load your solutions and walk away
All antibody and washing steps are automated for you
Sequential lateral flow controls the release of each antibody and wash solution
No electricity or battery required
Compatible with HRP- and AP-conjugated antibodies
How iBind works
The iBind™ Western System is an automated western processing device that employs sequential lateral flow (SLF) technology. SLF allows for the timely release of primary and secondary antibodies through mechanical pressure applied from the device onto the iBind™ Card. Glass fiber embedded in the iBind™ Card allows the antibody and wash solutions to flow at a constant rate over the western membrane. A wick attached to one end of the iBind™ Card pulls liquid across the card and membrane.
Get your iBind™ Western System Starter Kit now for only $999
Take advantage of faster, more sensitive and consistent western blots. In addition to the iBind™ Western Ssytem, the iBind™ Starter Kit provides cards and a solution kit, so you can get started right away. With the starter kit, you'll get:
Superior western performance compared to manual blotting
iBind™ delivers greater sensitivity for many monoclonal and polyclonal antibodies as compared to manual methods. Figure 1 compares the level of sensitivity for the iBind system versus manual western methods.
Combine the iBind western system with Novex highly specific primary and secondary antibodies to achieve clean western blots that only light up your target protein.
Automated processing provides better blot-to-blot consistency with CVs less than 5% vs. Manual method CVs of 13%.
Figure 1. Western blots processed on the iBind™ device show superior sensitivity compared to western blots processed manually. (A–B) Western blots with phosphorylated Akt (left to right: 30 µg–500 ng cell extract load) were processed either on the iBind™ device or using standard manual western processing protocols as specified by the antibody manufacturer (monoclonal anti–phospho-Akt[Thr308] (C31E5E) primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind™ device detected phospho-Akt in 500 ng of cell extract, while the target was detected in 4 µg on the manually processed blot. (C–D) Western blots with cell lysate expressing CREB (left to right: 30 µg–1 µg cell lysate load) were processed either on the iBind™ device or using standard manual western processing protocols as specified by the antibody manufacturer (polyclonal anti-CREB primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind™ system detected CREB in 6 µg of cell lysate, while 10 µg of lysate was needed to detect CREB on the manually processed blot. For all blots, proteins were separated using the Bolt® Gel Electrophoresis System and transferred to PVDF membranes using the iBlot® 7-Minute Blotting System.
Faster than manual western blotting methods
Runs in about 2.5 hours, delivering improved western quality compared to longer protocols
Compresses lengthy, overnight antibody incubation times to within 2.5 hours
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