iBind™ Western system

The iBind™ Western System is an automated western processing device. It’s hands-free, reproducible, and compatible with all western detection protocols.

  • Allows you to load your solutions and walk away
  • All antibody and washing steps are automated for you
  • Sequential lateral flow controls the release of each antibody and wash solution
  • No electricity or battery required
  • Compatible with most secondary antibodies including HRP, AP, Alexa Fluor®, and IRDye® (LI-COR®) conjugated antibodies

 

Western processing with the iBind™ System

The iBind™ Western System is an automated western processing device that employs sequential lateral flow (SLF) technology. SLF allows for the timely release of primary and secondary antibodies through mechanical pressure applied from the device onto the iBind™ Card. Glass fiber embedded in the iBind™ Card allows the antibody and wash solutions to flow at a constant rate over the western membrane. A wick attached to one end of the iBind™ Card pulls liquid across the card and membrane.

Superior western performance compared to manual blotting
  • The iBind™ System offers greater sensitivity compared to manual methods for many monoclonal and polyclonal antibodies. Figures 1 and 2 compare the level of sensitivity for the iBind™ System versus manual western methods.
  • Combine the iBind™ Western System with highly specific Novex® primary and secondary antibodies to achieve cleaner western blots.
  • Automated processing provides improved blot-to-blot consistency with CVs typically less than 5% (compared to manual processing that can have CVs of 13%).
  Figure 1. Western blots processed on the iBind™ device show superior sensitivity compared to western blots processed manually. (A–B) Western blots with phosphorylated Akt (left to right: 30 µg–500 ng cell extract load) were processed either on the iBind™ device or using standard manual western processing protocols as specified by the antibody manufacturer (monoclonal anti–phospho-Akt[Thr308] (C31E5E) primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind™ device detected phospho-Akt in 500 ng of cell extract, while the target was detected in 4 µg on the manually processed blot. (C–D) Western blots with cell lysate expressing CREB (left to right: 30 µg–1 µg cell lysate load) were processed either on the iBind™ device or using standard manual western processing protocols as specified by the antibody manufacturer (polyclonal anti-CREB primary antibody; HRP-conjugated anti-rabbit secondary antibody). The blot processed with the iBind™ system detected CREB in 6 µg of cell lysate, while 10 µg of lysate was needed to detect CREB on the manually processed blot. For all blots, proteins were separated using the Bolt® Gel Electrophoresis System and transferred to PVDF membranes using the iBlot® 7-Minute Blotting System.
Figure 2. iBind™ device vs. manual western blotting with fluorescent-based detection of c-Jun and Phospho-Stat3. Western blots with cell lysates expressing phospho-Stat3 and phoshpo-c-Jun (left to right: 30 μg–120 ng lysate) were processed either on the iBind™ device or using standard manual western processing protocols as specified by the antibody manufacturer (monoclonal phospho-Stat3 (Tyr705) (M9C6) mouse primary antibody and monoclonal c-Jun (60A8) rabbit primary antibody); A and C: Alexa Fluor® 680 goat anti-rabbit secondary antibody and Alexa Fluor® 790 goat anti-mouse secondary antibody. B and D: IRDye® 680LT goat anti-rabbit secondary antibody and IRDye® 800CW goat anti-mouse secondary antibody. Blots processed with the iBind™ device detected target proteins at lower levels than the manually processed blots: phospo-Stat3 in 235 ng of cell lysate and phospho-c-Jun in 470 ng of cell lysate (when processed with the Alexa Fluor® secondary antibodies); and detected phospo-Stat3 in 235 ng of cell lysate and phospho-c-Jun in 470 ng of cell lysate (when using LI-COR® IRDye® secondary antibodies). For all blots, proteins were separated using the Bolt® Gel Electrophoresis System and transferred to nitrocellulose membranes using the iBlot® 2 Dry Blotting System.
Faster than manual western blotting methods
  • Runs in about 2.5 hours, offering improved western blotting results compared to longer protocols
  • Compresses lengthy, overnight antibody incubation times to approximately 2.5 hours
  • Rapid 15-minute set up time

Learn more about the iBind™ Western System

Ordering Information

Sku Name Size Price Qty
SLF1000 iBind™ Western Device 1 device USD 900.00
SLF1001 iBind™ Window Cover 1 each USD 15.00
SLF1010 iBind™ Cards 10 cards USD 120.00
SLF1019 iBind™ Fluorescent Detection (FD) Solution Kit 1 kit USD 75.00
SLF1020 iBind™ Solution Kit 1 kit USD 60.00
SLF1000S iBind™ Western Starter Kit 1 kit USD 999.00
SLF10004PK iBind™ Western Device 4 devices USD 2,700.00
SLF1021 Novex® AP Mouse Chemiluminescent Detection Kit 1 kit USD 100.00
SLF1022 Novex® AP Rabbit Chemiluminescent Detection Kit 1 kit USD 100.00
626520 Goat Anti-Mouse IgG (H+L) - HRP 1 mL USD 119.00
656120 Goat Anti-Rabbit IgG - HRP 1 mL USD 119.00
WP20005 Novex® ECL Chemiluminescent Substrate Reagent Kit 2 x 125 mL USD 210.00
A11375 Alexa Fluor® 790 Goat Anti-Mouse IgG (H+L) 0.5 mL USD 149.00
A21109 Alexa Fluor® 680 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed 0.5 mL USD 199.00