Life Technologies makes blotting easier and more convenient by providing a variety of Novex® pre-cut, pre-assembled western blot membrane/filter-paper sandwiches that perfectly fit mini-gels, E-PAGE™ gels, and comparable size gels. PVDF (polyvinylidene difluoride) and nitrocellulose, membranes are available in different pore sizes and dimensions for different application needs. A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces so finding the optimal membrane may require experimenting with your specific protein on different membranes. Below are some basic guidelines to help you choose from Invitrogen™ western blot membranes available from Life Technologies.

Choosing Your Western Blot Membrane

Membrane Type Applications & Uses* Re-probe Characteristics Binding Interaction
Nitrocellulose Membranes WB-low mol wt proteins (<20 kDa), S,N-nucleic acids (<300 bp), AA, DSB Cannot Be Striped & Reprobed Hydrophobic and Electrostatic
iBlot® Transfer Stack, Nitrocellulose Membrane
WB-low mol wt proteins (<20 kDa) Cannot Be Striped & Reprobed Hydrophobic and Electrostatic
PVDF Membrane WB, P, AA, SPA Can Be Striped & Reprobed Hydrophobic
Invitrolon™ PVDF Membrane WB- proteins > 10 kDa, P, AA Can Be Striped & Reprobed Hydrophobic
iBlot® Transfer Stack, PVDF Membrane WB Can Be Striped & Reprobed Hydrophobic
*WB=Western Blot Transfer, S=Southern Transfer, N=Northern Transfer, AA=Amino Acid Analysis, DBS=Dot/Slot Blotting, P=Protein Sequencing, SPA=Solid Phase Assay Systems.