Nitrocellulose membrane is a high quality membrane ideal for blotting of proteins and nucleic acids. The major features of the nitrocellulose membrane available from Invitrogen include:

  • Composed of 100% pure nitrocellulose to provide high-quality transfer
  • Contains no support fabric or detergents
  • Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, fluorescence, or radiolabeling
  • Provides high-sensitivity with low background
  • Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich for convenience

Stripping and reprobing of nitrocellulose membranes is not recommended. For experiments requiring re-use please see our PVDF membranes.





Figure 1. Nitrocellulose blot of E-PAGE™ Gel developed with WesternBreeze® Kit using Nitrocellulose Membranes from Invitrogen. MagicMark™ XP Western Protein Standard (5 µL) was loaded into each well of an E-PAGE™ 48 8% Gel and transferred to a pre-cut 0.2 µm nitrocellulose membrane. The blot was probed with 1:1000 Anti-Xpress™ Antibody and detected using the WesternBreeze® Chemiluminescent (Panel A) or WesternBreeze® Chromogenic (Panel B) Immunodetection kit (anti-mouse).

Ordering information

Sku Name Size Price Qty
LC2010 Blotting Filter Papers, 2.5 mm thickness, 7.5 cm x 8.4 cm 50 sheets USD 52.00
LC2008 Blotting Filter Papers, 2.5 mm thickness, 8.6 cm x 13.5 cm 50 each USD 78.50
LC2009 Nitrocellulose/Filter Paper Sandwich, 0.2 µm pore size, 8.5 cm x 13.5 cm 16⁄pack USD 186.00
LC2006 Nitrocellulose/Filter Paper Sandwich, 0.45 µm pore size, 8.5 cm x 13.5 cm 16⁄pack USD 186.00
LC2000 Nitrocellulose Pre-Cut Blotting Membranes, 0.2 µm pore size 20⁄pack USD 154.00
LC2001 Nitrocellulose Pre-Cut Blotting Membranes, 0.45 µm pore size 20⁄pack USD 154.00
IB301032 iBlot® Transfer Stack, nitrocellulose, mini 3 x 10 sets USD 372.00
IB301031 iBlot® Transfer Stack, nitrocellulose, regular size 3 x 10 sets USD 462.00
IB301002 iBlot® Transfer Stack, nitrocellulose, mini 10 sets USD 149.00
IB301001 iBlot® Transfer Stack, nitrocellulose, regular size 10 sets USD 184.00
LP0001 NuPAGE® Large Protein Blotting Kit 1 kit USD 842.00
IB7710 Novex® Reversible Membrane Protein Stain Kit 1 kit USD 87.75
LP0002 NuPAGE® Large Protein Staining Kit 1 kit USD 692.00
LP0003 NuPAGE® Large Protein Sensitive Staining Kit 1 kit USD 786.00