One of the key steps in the western blot workflow is the transfer of proteins from the polyacrylamide gel after electrophoresis to the nitrocellulose or polyvinylidene difluoride (PVDF) membrane so that specific proteins can be detected using immunodetection techniques.

Methods for achieving this protein transfer include capillary transfer or electrophoretic transfer. We have developed electrophoretic transfer systems using wet, semi-dry, and dry methods outlined below.

Which western transfer system is right for you?

Traditional wet transfer in 1–2 hours Traditional wet transfer in 1 hour Semi-dry transfer for high efficiency Dry transfer in 7 minutes
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XCell II™ Blot Module

Bolt® Mini Blot Module

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Novex® Semi-Dry Blotter

iBlot® 2 Gel Transfer Device

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Transfer time 60–120 min 60 min 30–60 min 7 min
Capacity of device 1–2 mini gels 1 mini gel (per module) or 2 mini gels (two modules per tank) 2 mini gels or
1 midi gel
2 mini gels or
1 midi gel
Blotting area 9 x 9 cm 9 x 9 cm 21 x 21 cm 8.5 x 13.5 cm
Requires transfer buffer Yes (1,000 mL) Yes, (200–400 mL per module) Yes (500 mL) No
Power supply External External External Internal

Transfer buffers

  • Convenient pre-mixed buffers ensure high quality, consistent results
  • Made with high-quality reagents and strict quality control
  • Available in concentrated form for dilution prior to use

See Pre-Mixed Buffers for Protein Electrophoresis