Bolt™ Mini Blot Module

Complete your Bolt™ system with the innovative Bolt™ Mini Blot Module that conveniently fits into the chambers of the Bolt™ Mini Gel Tank. The Bolt™ Mini Blot Module is a wet-transfer device that allows you to easily transfer proteins from mini gels to membranes. The tank accommodates one blot module per chamber, or a total of two blot modules with side-by-side layout. This affordable, leak-resistant module requires less transfer buffer than other commercially available transfer systems, and the constant resistance across the blotting electrodes helps ensure uniform field strength for highly efficient western transfers.

Your transfer can be configured with either nitrocellulose or PVDF (polyvinylidene difluoride) membranes for western blotting.

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Bolt™ Western Packs

The Bolt™ Western Packs provide all of the reagents and buffers you will need to run your western transfer.

Each pack contains:

  • 2 Bolt™ Mini Blot Modules
  • 125 mL Bolt™ Transfer Buffer (20X)
  • 15 mL Bolt™ Antioxidant
  • Blotting membrane/filter paper sandwich, 20/pack
    • Nitrocellulose 0.2 µm in Western Pack Cat. No. B1000A
    • PVDF 0.2 µm in Western Pack Cat. No. B1000B

Convenient, Reliable Western Transfer

The Bolt™ Mini Blot Module conveniently fits into the Bolt™ Mini Gel Tank and is designed to make your western transfers simple and easy to perform.

  • Universal module design—allows modules to fit in either chamber of the tank, simplifying the transfer setup
  • Unique gasket seal—helps prevent buffer leakage so there is no mess during setup of your western transfer
  • 1/2 inch buffer chamber—requires only half the volume of methanol-based transfer buffer and helps save you money
  • Standard 60 min transfer protocol—accelearates your western workflow so you can get results faster
  • Robust electrodes, sturdy steel plates—for highly efficient and reliable western transfers

Western Blotting With High-Quality Reagents

For downstream western blot analyses, be sure to use high-quality, high-specificity secondary antibodies to deliver the best-quality western blot. All Novex® secondary antibodies are specially purified to reduce cross-reactivity with bovine antigens. Available secondary antibody reagents include goat anti-rabbit and goat anti-mouse polyclonal alkaline phosphatase conjugates.

High-Performance Western Transfer

The Bolt™ Bis-Tris Plus electrophoresis and transfer system preserves protein integrity due to its neutral-pH formulation and mild sample preparation conditions, so you can minimize the protein degradation commonly observed with Tris-glycine gels.

  • Excellent band quality—attributed to Novex® Bis-Tris Plus chemistry, which is designed to deliver sharp, straight, uniform bands (see figure)
  • Greater western sensitivity—optimized for greater western sensitivity with consistency across dilution series
  • Superior protein integrity and cleaner blots—western blots from Bolt™ gels show strong signals from full-length, undegraded proteins, compared to western blots from Tris-glycine gels that show a greater degree of protein degradation (see figure)

A western blot of a Bolt™ gel shows clean, sharp protein signals corresponding to only full-length proteins, whereas a western blot of a Bio-Rad® TGX™ gel shows multiple low-molecula- weight degradation products. Protein kinases implicated in cancer (IKKB, HCK, EPHB3, MAPK14, FLT1, and DDR2) were analyzed on (A) a Bolt™ Bis-Tris Plus gel and (B) a Bio-Rad® TGX™ Tris-glycine gel. Protein samples were prepared for electrophoresis according to each manufacturer’s protocol. The purified kinases (50 ng each) as GST fusion proteins, along with MagicMark™ XP protein standard and purified recombinant GST, were loaded in a Bolt™ 4-12% gel and a Bio-Rad® TGX™ 4-20% gel. The samples were separated and transferred to PVDF membranes using the Bolt™ Mini  Blot Module for the Bolt™ gels or on the Bio-Rad transfer system. Blot detection was performed using an anti-GST antibody and a WesternBreeze® Chemiluminescence Detection Kit. The membranes were then imaged using an LAS-1000 system (FujiFilm) with an exposure time of 1 minute.

Bolt™ Module Videos

How to Perform a Western Transfer

 

How to Run the Bolt Pre-Cast Protein Gel

Ordering Information

Sku Name Size Price Qty
B1000 Bolt® Mini Blot Module 1 unit USD 275.00
B1000A Bolt® Western Pack A (Nitrocellulose) 1 kit USD 590.00
B1000B Bolt® Western Pack B (PVDF) 1 kit USD 590.00
BT0005 Bolt® Antioxidant 15 mL USD 24.00
BT0006 20X Bolt™ Transfer Buffer 125 mL USD 21.00
BT00061 20X Bolt™ Transfer Buffer 1 L USD 149.00

Bolt™ Western Packs