Western Blot Buffers & Accessories
Below you will find a diverse selection of buffers, reagents, kits and accessories to help you get consistent, reliable results for your western blot analysis. These products can be used with all of our western blot systems.
Blotting Buffers, Blocking Reagents and Protein Transfer Buffers
Western Blotting Roller and Incubation Tray
The Blotting Roller is an 8.6 cm wide roller designed for easy and efficient removal of bubbles when assembling gel/membrane sandwiches for blotting. These include our E-PAGE™ Gels and our NuPAGE® and Novex® Protein Gels. The roller consists of a Del-rin roller attached to a stainless steel handle. One Blotting Roller is supplied per pack.
The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with western immunodetection or blot stains. Each pack of Incubation Trays provides 8 plastic trays and 8 lids.
Blotting Roller & Incubation Tray Ordering Information
Novex® Reversible Membrane Protein Stain Kit
The Novex® Reversible Membrane Protein Stain Kit allows complete, reversible staining of proteins on nitrocellulose and PVDF membranes, providing highly sensitive protein band detection (<10 ng bovine serum albumin) with minimal background. The staining procedure is complete in less than 10 minutes, resulting in blue protein bands and provides higher sensitivity than the standard Ponceau S staining method. The staining reaction is fully reversed in 5 additional minutes.
Subsequent western blot detection is unaffected by the staining and erasing process, and in some cases higher sensitivity is achieved.
|Kit Component||Item Amount*|
|Stain Solution||167 mL|
|Destain Solution||150 mL|
|Eraser (contains sodium carbonate)||150 mL|
|*Sufficient amount is supplied for reversible staining and erasing of 10 (8 cm x 8 cm) membranes|
NuPAGE® Large Protein Blotting Kit
The NuPAGE® Large Protein Blotting Kit is ideal for Western blotting of high molecular weight proteins. The kit includes precast protein gels, pre-made buffers, and pre-assembled nitrocellulose membrane/filter paper sandwiches to ensure consistent results and eliminates the need to prepare reagents. A pre-stained protein standard (HiMark™) specifically designed for analysis of high molecular weight proteins is also included to allow you to easily monitor the protein transfer. This kit is designed for use with the XCell II™ Blot Module. Sufficient reagents are included to run and blot 20 mini-gels.
|HiMark™ Pre-Stained High Molecular (HMW) Weight Protein Standard||250 μL|
|NuPAGE® Novex 3-8% Tris-Acetate Gel (1.0 mm, 10 wells)||20 gels|
|NuPAGE® Tris-Acetate SDS Buffer Kit||1 kit|
|NuPAGE® Transfer Buffer (20X)||1 L|
|Nitrocellulose Membrane/Filter Sandwiches (0.45 μm)||20 sandwiches|
Novex® and NuPAGE® Kits Ordering Information
Power Supplies for Western Blot Analysis
ZOOM® Dual Power Supply
The ZOOM® Dual Power Supply is a microprocessor-controlled programmable power supply capable of running both high-voltage/low-current and high-current/low-voltage applications concurrently.
- The high-voltage/low-current section is ideal for sample fractionation, IPG strips, IEF, and DNA sequencing applications.
- The low-voltage/high-current section is for SDS-PAGE, native PAGE, second-dimension SDS-PAGE, DNA/RNA electrophoresis, and blotting applications.
Four sets of output jacks on each side of the unit allow for multiple apparatuses to run simultaneously, offering greater workflow efficiency and higher sample throughput.