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  • Custom and pre-defined collections of Ambion Silencer® Select siRNAs to popular human gene classes/targets provided in 96- or 384-well plates
  • Designed with an algorithm that yields better, more consistent knockdown
  • Novel chemical modifications reduce off-target effects by up to 90%
  • Up to 100-fold more potent than currently available siRNAs
  • Three individual siRNAs per target provided in a convenient 96-well format
  • Demonstrated improvements in consistency and reliability of phenotypic results

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What are Silencer® Select siRNAs?

Silencer® Select siRNAs are the next generation Ambion® siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. In siRNA screening applications, the result is fewer false negatives due to poor silencing and fewer false positives due to off-target effects. In other words, with Silencer® Select siRNAs you get cleaner, more consistent data from your primary screen, so you expend less time and resources to find true, validated targets. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data. Learn more about Silencer® Select siRNAs

You can obtain the benefits of Ambion® Silencer® Select siRNAs in a convenient 96 or 384-well plate format plated to your specifications. These siRNA libraries enable medium- and high- throughput analysis of phenotypes resulting from inhibition of sets of targets from specific functional classes or your favorite list of targets.

Custom Silencer® Select siRNA libraries

The ultimate in flexibility!

Do you have a favorite list of genes you would like to target with a collection of siRNAs? We can prepare a custom Silencer® Select siRNA Library to any set of human genes. The minimum order is only 36 siRNAs. All you need to supply is a list of genes or transcripts identified by NCBI Entrez Gene ID or RefSeq mRNA accession number. With this user-friendly option, you can specify the order of the siRNAs on the plate, as well as the amount of siRNA per order (1 nmol minimum), number of aliquots of each plate, and the number of siRNAs per target. siRNAs can be provided individually or pooled.

Pre-defined Silencer® Select siRNA libraries

Three individual siRNAs per target provided in a convenient 96-well format
See the Silencer® Select Pre-designed siRNAs guarantee.*

Human Genome siRNA Library

Increasingly, researchers are performing genome-scale surveys of gene function. The Silencer® Select Human Genome siRNA Library V4 is ideal for this purpose. Three unique, non-overlapping siRNAs are provided for each of 21,585 human targets. The siRNAs targeting the “druggable” portion of this library are arranged by gene functional class to enable easy screening of important gene subsets such as kinase, phosphatase, GPCR, etc. This siRNA library is supplied in 384-well plates.

Human Druggable Genome and Human Extended Druggable Genome siRNA Libraries

With siRNAs targeting the most therapeutically relevant human genes and conveniently grouped by target gene class, the Silencer® Select Human Druggable Genome siRNA Library V4 and Silencer® Select Human Extended Druggable Genome siRNA Library V4 are popular choices for many researchers. The druggable genome set targets 9032 genes, whereas the extended druggable genome collection targets those genes plus the "Extension Set" for a total of 10,415 genes, including transcription factors. Please contact us for the complete list of targets. These libraries are available in both 96- and 384-well plate formats.

Human Kinase siRNA Library

Because of their importance in cell signaling and many biological pathways, kinases are key drug targets and subjects of intense scrutiny. The Silencer® Select Human Kinase siRNA Library V4 targets 710 human kinases with three individual Silencer® Select siRNAs per gene. This siRNA library enables systematic, yet cost effective, RNAi studies of these key cell regulators. Validated siRNAs are included where available. The Silencer® Select Human Kinase siRNA Library V4 is provided in 96-well plates.

Human Phosphatase siRNA Library

Along with kinases, phosphatases are important regulators of biological pathways and cell signaling cascades. The Silencer® Select Human Phosphatase siRNA Library V4 targets 298 human phosphatases with three individual siRNAs per gene supplied in 96-well plates. This library is the ideal companion to the Silencer® Select Kinase siRNA Library V4, enabling more detailed studies of biological pathways.

Human GPCR siRNA Library

G-protein–coupled receptors, or GPCRs, are key components of many signal transduction pathways and also represent an important class of druggable genes. The Silencer® Select Human GPCR siRNA Library V4 targets 380 non-olfactory human GPCRs with three individual Silencer® Select siRNAs per target supplied in 96-well plates.

Human Protease, Nuclear Hormone Receptor, and Ion Channel siRNA Libraries

Focused siRNA sets to human proteases, ion channels, and nuclear hormone receptors provide a cost- effective means of studying these important regulators of cellular function. These siRNA libraries are each supplied in 96-well plates. The Silencer® Select Human Protease siRNA Library V4 features 3 siRNAs per target for 494 human proteases. The Silencer® Select Human Nuclear Hormone Receptor siRNA Library V4 includes 3 siRNAs per target for 47 nuclear hormone receptors. The Silencer® Select Human Ion Channel siRNA Library V4 features 3 siRNAs per target for 338 human ion channels.

Individual siRNAs for enhanced data reliability

Silencer® Select siRNA Libraries feature three individual siRNAs for each target. Screening with three siRNAs per gene significantly decreases both false positive and false negative rates, resulting in enhanced confidence in RNAi screening data, reduced chance of missing important genes, and less time spent following up on false positive hits from the screen.

These libraries contain 0.25 nmol of each siRNA, sufficient for 500 transfections (at 5 nM siRNA, 100 µL transfection volume). The siRNAs are supplied dried into the wells of 96 well plates. One column of each 96 well plate is left empty to allow for the addition of controls at the time of use. The libraries are delivered with full siRNA sequence information, as well as the exon targeted and each targeted gene's symbol, full name, aliases, RefSeq number, and Entrez Gene ID, plus results from any validation experiments performed using the included siRNAs.

Silencer® Select siRNA Libraries to these or any other list of human genes can also be provided with 1, 2, or 5 nmol of each siRNA. Custom formatting is available with these larger sizes. If you would like a gene list or other information, or would like to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAilibraries@lifetech.com


*Silencer® Select Pre-designed siRNAs guarantee

Life Technologies guarantees that when you purchase two Silencer® Select Pre-designed siRNA to the same target, then those two siRNAs will silence the target mRNA by 70% or more. To qualify for the guarantee, siRNA's must have been transfected at ≥5 nM and mRNA levels detected 48 h post transfection. Real-Time RT-PCR is recommended but not required for this application. Customers must also show sufficient knockdown with a positive control siRNA to demonstrate transfection efficiency. If the guaranteed level of knockdown is not observed and an appropriate positive control is successful, a new Silencer® Select siRNA sequence will be synthesized free of charge. This guarantee does not extend to any replacement product.