ベクターベースRNAi

RNA interference (RNAi) is the best way to effectively knock down gene expression and study protein function in a wide range of cell types.  Using vectors to perform RNAi experiments greatly expands experimental possibilities beyond traditional small/short interfering RNAs (siRNAs). 

Vector technologies allow you to:

  • Achieve transient or stable target knockdown
  • Perform RNAi in any cell type, even hard to transfect, primary and non-dividing cells
  • Regulate gene inhibition with inducible RNAi expression
  • Select for a pure population of cells stably expressing an RNAi sequence
  • Control gene expression in vivo with tissue specific promoters

Table 1. Comparison of RNAi vector technologies.

BLOCK-iT™ Pol II miR RNAi BLOCK-iT™ shRNA
Pol II expression system Pol III expression system
Polycistronic expression of miRNAs (chaining) Single shRNA expression per promoter
Co-expression of GFP allows expression to be tracked Only delivery can be tracked (with separate expression cassette)
Compatible with most Gateway® DEST vectors Compatible with BLOCK-iT™ DEST Vectors
Best design success (>70%) Moderate design success (~50%)

Table 2. Life Technologies RNAi vector products.

Product Type Description Online Ordering
BLOCK-iT™ miR RNAi Select Pre-designed miR RNAi vector inserts for any organism Vector inserts: BLOCK-iT™ RNAi Express
Vector backbones: Invitrogen’s online catalog
BLOCK -iT™ Pol II miR Validated miRNA Vector DuoPak Validated miR RNAi constructs to human kinase genes Glycerol stocks: BLOCK-iT™ RNAi Express
Custom BLOCK-iT™ Pol II miR RNAi Vectors Custom miR RNAi vectors for any organism Vector inserts: BLOCK-iT™ RNAi Designer
Vector backbones: Life Technologies online catalog
BLOCK-iT™ shRNA Vectors Custom shRNA vectors for any organism Vector inserts: BLOCK-iT™ RNAi Designer
Vector backbones: Life Technologies online catalog
Controls for RNAi Vectors Transfection, positive and negative controls for every RNAi experiment Life Technologies online catalog

Ambion® pSilencer™ siRNA Expression Vectors
The pSilencer siRNA Expression Vector line includes several classic plasmid vectors with U6 (RNA polymerase III) promoters.  These systems include pre-cut vector, a negative control vector, and a positive control insert.  Please note that these vectors do not include cells and are not Gateway compatible.

Expression vector
Vector type Application
pSilencer 2.1-U6 Neo
shRNA vector (not Gateway compatible) Transient or stable expression of shRNA in transfected cells.  Select for stable expression with Neomycin.
pSilencer 2.1-U6 Hygro
shRNA vector (not Gateway compatible) Transient or stable expression of shRNA in transfected cells.  Select for stable expression with Hygromycin.
pSilencer 2.1-U6 Puro shRNA vector (not Gateway compatible) Transient or stable expression of shRNA in transfected cells.  Select for stable expression with Puromycin.

Custom Services
Need help with your RNAi research? Contact us for a consultation and quote today.

Questions
Send your questions to our top researchers at RNAiresearcher
@thermofisher.com