Microbial Sequencing by Ion Torrent™ Next-Generation Sequencing
FROM OUTBREAK ANALYSIS TO SURVEILLANCE
Powered by the Ion PGM™ System now with 400 base pair sequencing for long, accurate reads.
Rapid, comprehensive, and broad-range research analysis of microbial populations using the Ion PGM System.
The evolution of Ion semiconductor sequencing has enabled researchers to take advantage of increased throughput, higher accuracy, and longer reads to produce rapid and accurate sequencing of microbes with streamlined sample preparation and a simple and optimized data analysis workflow. In addition, the Ion PGM™ System has been critical for facilitating a rapid response during disease surveillance, investigating outbreaks, and determining disease etiology.* Downstream of microbial sequencing, data analysis methods include de novo and reference-guided assembly and multi-locus sequence typing (MLST) for bacterial identification tests or typing of microbial strains.
Read application notes and peer-reviewed publications to learn how the Ion PGM™ System has empowered advances in microbes sequencing.
Download a data set generated on the Ion PGM™ System, and see the results for yourself.
Learn how the Ion PGM™ System has enabled customers in their microbiology research.
Microbial Sequencing Application Notes, Literature and Publications
Application Notes and Literature
Microbial Solutions Brochure
16s rRNA Gene Sequencing on the Ion PGM™ System application note
Discover how German scientists leveraged the Ion PGM™ Sequencer to get answers when faced with a serious public health outbreak (shiga toxin-producing E. coli outbreak in northern Germany)
The Ion PGM™ System, with 400-base read length chemistry, enables routine high-quality de novo assembly of small genomes
Ion PGM™ System and PathAmp™ FluA Reagents—Influenza A whole-genome sequencing
The Ion PGM™ System is cited in more than 40 peer-reviewed publications about small genome sequencing, making it the leading system for de novo assembly of small genomes.
Petrof, E., et al. (2013). Stool substitute transplant therapy for the eradication of Clostridium difficile infection: 'RePOOPulating' the gut Microbiome 2013, 1:3. DOI: 10.1186/2049-2618-1-3
Hassan, S. S., et al. (2012). Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology Stand Genomic Sci 7(2): 189-199. DOI: 10.4056/sigs.3066455
Yergeau, E., et al. (2012). Next-generation sequencing of microbial communities in the Athabasca River and its tributaries in relation to oil sands mining activities Appl Environ Microbiol 78(21): 7626-7637. DOI: 10.1128/AEM.02036-12
Microbial Sequencing videos
For Research Use Only. Not for use in diagnostic procedures.
*Mellmann A, Harmsen D, Cummings CA et al. (2011) Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology. PLoS One 6, e22751; Rohde H, Qin J, Cui Y et al. (2011) Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4. N Engl J Med 365, 718-724; Sherry NL, Porter JL, Seemann T et al. (2013) Outbreak investigation using high-throughput genome sequencing within a diagnostic microbiology laboratory. J Clin Microbiol. 2013 Feb 13. [Epub ahead of print ]