Simplified sequencing for challenging disease agents such as influenza A.

Application Note

Read about complete influenza A genome sequencing on the Ion PGMnext-generation sequencer.

Influenza A Typing Workflow

Complete influenza A genome sequencing in about a day from RNA through sequencing.

Influenza Network Poster

See how the PathAmp™ Flue A reagents were used on the Ion PGM™ System to detect Influenza A subtypes.

Influenza A Typing on the Ion PGM System

A major challenge for microbiologists and virologists is the prediction of patterns of evolution and emergence of disease agents. RNA viruses like influenza A share the biological feature of high genetic variability, which causes them to appear as clouds of mutants. Influenza A variants also emerge through antigenic shift within animal reservoirs, such as pigs and birds. The World Health Organization estimates that seasonal influenza A is responsible for epidemics that cause approximately three to five million cases of severe illness, and 250,000 to 300,000 deaths annually.

Ion semiconductor sequencing has been paired with PathAmp™ FluA reagents to provide a streamlined workflow for complete influenza A genome sequencing. PathAmp™ FluA reagents are a set of highly specific, universal influenza primers combined with a high-fidelity master mix for the amplification of all eight influenza A genomic segments in a single tube. The reaction produces DNA amplicons which can then be used for Ion PGM™ System sequencing to deliver highly accurate influenza A typing in under a day. As a result, it enables more effective influenza surveillance, helps refine the selection of vaccine strains, and improves predictions of future antigenic characteristics.

Viral Typing Workflow

Viral Typing Application Notes, Literature and Publications

Application Notes and Literature

Microbial Solutions Brochure
Complete influenza A genome sequencing on the Ion PGMnext-generation sequencer.