Chimerism is a term used to describe the occurrence of genetically distinct cell types in a single organism, which can result from transfusion or transplantation or can be inherited (e.g., in plants). In the case of a bone marrow transplant, the relative amounts of donor and recipient cells can be used to determine if engraftment has taken place.
Chimerisms can be monitored via sensitive PCR-based methods and analysis of STR markers. The sensitivity, improved data quality, and precision of the 3500 Series Genetic Analyzers combined with our five-color chemistry and pre-optimized run conditions create the ideal solution for STR analysis.
Step-by-Step Guide to Chimerism
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.
Recommended Products: DNA Isolation
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis.
Recommended Products: Prepare Sample for Analysis
To learn more about our GeneScan Size standards used in sizing experiments please see our table below.
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.
Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You?
Analyze the microsatellite profile. Appearance of numerous extra alleles in the microsatellite profile indicates a genome instability.
Which Data Analysis Software Is Right for You?
Highlighted products for Chimerism Analysis:
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.