Microsatellite Short Tandem Repeat (STR) Analysis
Variable nucleotide tandem repeats (VNTRs) are repeating sequences of multi-base segments of DNA. If the repeat is equal to or less than 6 bases, NTRs are named microsatellites, also known as short tandem repeats (STRs). One common example of a microsatellite is a (CA)n repeat, where n varies between alleles. Because microsatellites are polymorphic DNA loci present throughout the genome, microsatellite genotyping is a widely accepted tool for a variety of research applications such as linkage mapping studies, association studies, and identification of organisms.
Our five-dye chemistry increases the number of microsatellite markers that can be run in a single capillary, substantially improving the throughput.
Step-by-Step Guide to Microsatellite STR Analysis
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.
Recommended Products: DNA Isolation
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis.
Recommended Products: Prepare Sample for Analysis
To learn more about our GeneScan Size standards used in sizing experiments please see our table below.
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.
Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You?
Highlighted Products for Microsatellite STR Analysis:
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.